Document 0048
 DOCN  M9610048
 TI    Japanese encephalitis virus-specific proliferative responses of human
       peripheral blood T lymphocytes.
 DT    9601
 AU    Konishi E; Kurane I; Mason PW; Innis BL; Ennis FA; Department of Medical
       Zoology, Kobe University School of; Medicine, Japan.
 SO    Am J Trop Med Hyg. 1995 Sep;53(3):278-83. Unique Identifier : AIDSLINE
       MED/96033014
 AB    The T lymphocytes play an important role in prevention and recovery from
       viral infections. To characterize T lymphocyte responses to Japanese
       encephalitis (JE) virus infections, we analyzed JE virus-specific T
       lymphocytes in peripheral blood mononuclear cells (PBMC) obtained from
       seven JE patients and 10 vaccinees who had received a
       formalin-inactivated, purified JE virus vaccine (Biken vaccine). These
       PBMC were examined for proliferative responses against live JE virus, a
       glutaraldehyde-fixed lysate of cells infected with JE virus, and
       extracellular particles (EPs; subviral membrane vesicles released from
       cells infected with recombinant vaccinia viruses encoding the JE virus
       premembrane and envelope proteins). Japanese encephalitis virus-specific
       T cell proliferation was demonstrated with PBMC from both patients and
       vaccinees after stimulation with infectious JE virus or the lysate of JE
       virus-infected cells. Proliferating PBMC included CD4+ T lymphocytes and
       CD8+ T lymphocytes in responses to either form of JE viral antigens.
       Responses to EPs were observed only with PBMC from some American
       vaccinees whose PBMC also responded to the virus and lysate. These
       results indicate that JE virus infection and immunization with an
       inactivated JE vaccine induce JE virus-specific CD4+ and CD8+ T memory
       lymphocytes that can be induced to proliferate by infectious JE virus
       and noninfectious JE antigens.
 DE    Antibodies, Viral/BLOOD  Antigens, Viral/IMMUNOLOGY  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY
       Encephalitis Virus, Japanese/*IMMUNOLOGY  Encephalitis,
       Japanese/*IMMUNOLOGY  Human  *Lymphocyte Transformation  Middle Age
       Phenotype  Radioimmunoprecipitation Assay  Support, Non-U.S. Gov't
       Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/*IMMUNOLOGY  Vaccines, Inactivated/IMMUNOLOGY  Viral
       Vaccines/*IMMUNOLOGY  JOURNAL ARTICLE

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