Document 0106
 DOCN  M9610106
 TI    Biological characterization and molecular cloning of murine C-type
       retroviruses derived from the TSZ complex from mainland China.
 DT    9601
 AU    Bundy LM; Ru M; Zheng BF; Cheng L; Pattengale PK; Portis JL; Fan H;
       Department of Molecular Biology and Biochemistry, University of;
       California at Irvine 92717, USA.
 SO    Virology. 1995 Oct 1;212(2):367-82. Unique Identifier : AIDSLINE
       GENBANK/M17739
 AB    Characterization of the SRS murine retrovirus complex, derived from the
       TSZ system of murine leukemia developed in China, was carried out. The
       initial stock contained XC+, NB-tropic virus (and possibly other
       viruses), and induced several neoplastic diseases in neonatally
       inoculated NIH Swiss mice: erythroid leukemia, myeloid leukemia (acute
       myeloblastic leukemia), and lymphoblastic lymphoma (both B- and
       T-lymphoid). In addition, approximately 30% of inoculated animals
       developed central nervous system disease--hindlimb paralysis or
       semilateral paralysis. Rescue of virus from the spleen of an animal with
       combined erythroid/myeloid leukemia, followed by endpoint dilution gave
       two stocks: 19-6 (XC+, NB-tropic) and 19-7 (XC-, NB-tropic). Both stocks
       induced erythroid and myeloid leukemia, and 19-6 induced CNS symptoms as
       well. Southern blot analysis indicated that the predominant viruses from
       the 19-6 and the 19-7 cultures were related, but different in the env
       region. An infectious virus molecular clone of provirus from 19-6 cells
       was obtained. The resulting cloned virus [SRS 19-6 murine leukemia virus
       (MuLV)] induced four kinds of leukemia: erythroid, myeloid, B-lymphoma,
       and T-lymphoma; in many cases, more than one tumor type was identified
       in the same animal. Such a broad spectrum of leukemias induced by a
       cloned MuLV is unusual. Flaccid hindlimb paralysis induced by SRS 19-6
       MuLV could be attributed to meningeal B-lymphoma. Immunofluorescent
       staining with a panel of env-specific monoclonal antibodies confirmed
       that the 19-6 and 19-7 viral stocks contained different viruses, which
       differed from previously characterized MuLVs. The viruses of the SRS
       complex may provide interesting reagents for investigations of
       MuLV-induced disease.
 DE    Animal  Animals, Newborn  Base Sequence  China  Cloning, Molecular  DNA,
       Viral/GENETICS  Genes, env/GENETICS  Leukemia Viruses,
       Murine/*GENETICS/PATHOGENICITY  Leukemia,
       Experimental/PATHOLOGY/*VIROLOGY  Lymphoma,
       Lymphoblastic/PATHOLOGY/VIROLOGY  Mice  Molecular Sequence Data
       Paralysis/VIROLOGY  Proviruses/GENETICS  Restriction Mapping
       Retroviridae Infections/PATHOLOGY/*VIROLOGY  Sequence Analysis, DNA
       Spleen/VIROLOGY  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Tumor Virus Infections/PATHOLOGY/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).