Document 0108
 DOCN  M9610108
 TI    Comparison of serological tests for the diagnosis of feline
       immunodeficiency virus infection of cats.
 DT    9601
 AU    Sibille P; Avrameas A; Moraillon A; Richardson J; Sonigo P; Pancino G;
       Strosberg AD; Institut Cochin de Genetique Moleculaire, Unite;
       d'Immunopharmacologie moleculaire et genetique des virus, CNRS; UPR 415,
       Paris, France.
 SO    Vet Microbiol. 1995 Jul;45(2-3):259-67. Unique Identifier : AIDSLINE
       MED/96037974
 AB    A new enzyme-linked-immunosorbent assay (ELISA) for the detection of
       antibodies to feline immunodeficiency virus was compared with previously
       described ELISAs. Serum samples from 184 infected or uninfected cats
       were tested using a whole virus lysate kit and ELISAs based on
       recognition of one of two synthetic peptides (P237 and P253) localized
       in the transmembrane domain of the viral envelope. The whole virus
       lysate commercial kit led to the detection of 6% false positive and 4.3%
       false negative sera. The ELISA based on peptide P253 gave no false
       positive result and failed to detect only one serum that was
       subsequently shown to be positive by radio-immunoprecipitation assay. A
       sandwich-ELISA test using Galanthus nivalis agglutinin, a lectin that
       specifically binds terminal mannose groups of the envelope proteins was
       used as a confirmatory test for equivocal results with peptide ELISA and
       gave similar results. This study indicates that recognition of P253
       could serve as a sensitive and specific test for the diagnosis of
       seropositivity to feline immunodeficiency virus, and moreover that the
       Galanthus nivalis ELISA could be useful in equivocal cases as a
       confirmatory test.
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/BLOOD  Cats  Comparative
       Study  Enzyme-Linked Immunosorbent Assay/METHODS/*VETERINARY  Feline
       Acquired Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY/  VIROLOGY
       Immunodeficiency Virus, Feline/IMMUNOLOGY  Molecular Sequence Data
       Sensitivity and Specificity  JOURNAL ARTICLE

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