Document 0148
 DOCN  M9610148
 TI    The neuropeptide substance P does not influence the migration of B, T,
       CD8+ and CD4+ ('naive' and 'memory') lymphocytes from blood to lymph in
       the normal rat.
 DT    9601
 AU    Heerwagen C; Pabst R; Westermann J; Center of Anatomy, Medical School of
       Hannover, Germany.
 SO    Scand J Immunol. 1995 Oct;42(4):480-6. Unique Identifier : AIDSLINE
       MED/96017850
 AB    Thoracic duct lymphocytes (TDL) continuously patrol through the body,
       facilitating immune responses at most sites. The neuropeptide Substance
       P might regulate immune responses by influencing the migration of TDL.
       Therefore, it was investigated whether Substance P affects the migration
       of thoracic duct B, T, CD8+ and CD4+ ('naive' and 'memory') lymphocytes
       from blood to lymph in vivo. Labelled TDL were either incubated with
       Substance P and then injected into normal rats, or incubated without
       Substance P and then injected into rats continuously receiving Substance
       P intravenously. The numbers of labeled B, T, CD8+ and CD4+ ('naive' and
       'memory') lymphocytes were determined in blood and thoracic duct lymph
       for 1 and 5 days, respectively. Neither the in vitro incubation with
       Substance P nor its in vivo application influenced the disappearance of
       any lymphocyte subset from the blood or its reappearance in the lymph.
       In addition, continuous intravenous application of the Substance P
       antagonist CP 96.345 did not alter the volume or the lymphocyte number
       of the efferent lymph. The present study indicates that the nervous
       system does not influence immune responses via Substance P by altering
       the migration pattern of B, T, CD8+ and CD4+ ('naive' and 'memory')
       lymphocytes.
 DE    Animal  B-Lymphocytes/*CYTOLOGY/DRUG EFFECTS  Blood
       Cells/CYTOLOGY/IMMUNOLOGY  Cell Movement/*DRUG EFFECTS  CD4-Positive
       T-Lymphocytes/*CYTOLOGY/DRUG EFFECTS  CD8-Positive
       T-Lymphocytes/*CYTOLOGY/DRUG EFFECTS  Flow Cytometry
       Lymph/CYTOLOGY/IMMUNOLOGY  Male  Rats  Rats, Inbred Lew  Substance
       P/*PHARMACOLOGY  Support, Non-U.S. Gov't  T-Lymphocytes/*CYTOLOGY/DRUG
       EFFECTS  JOURNAL ARTICLE

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