Document 0149
 DOCN  M9610149
 TI    Dysregulation of T helper cell cytokines in autoimmune prone NZB x NZW
       F1 mice.
 DT    9601
 AU    Lin LC; Chen YC; Chou CC; Hsieh KH; Chiang BL; Department of Pediatrics,
       College of Medicine, National Taiwan; University, China.
 SO    Scand J Immunol. 1995 Oct;42(4):466-72. Unique Identifier : AIDSLINE
       MED/96017848
 AB    Multifactorial involvement in the pathogenesis of autoimmune NZB/W F1
       mice has been well documented. To further elucidate the role of
       cytokines in the disease development of murine lupus, single spleen
       cells isolated from NZB/W F1 and non-autoimmune C57BL/6 mice were
       stimulated with T cell mitogens or anti-CD3 antibody at pre-determined
       optimal concentration. Supernatants were collected and assayed for
       production of cytokines including IL-2, gamma-IFN, IL-3, IL-4, IL-5 and
       IL-10. In both young and old mice, cytokine profiles by
       mitogen-stimulated T cells showed higher TH2 (type 2 T helper)
       cell-related cytokine production in NZB/W F1 mice compared to those in
       non-autoimmune C57BL/6 mice. In contrast, cytokines produced by TH1
       (type 1 T helper) cells, such as gamma-IFN and IL-2, were lower in NZB/W
       F1 mice by stimulation with either mitogen or anti-CD3 antibody. In
       addition, cytokine production at different time points also demonstrated
       decreased gamma-IFN and increased IL-4 levels by anti-CD3 stimulated
       splenic cells in autoimmune NZB/W F1 mice. Furthermore, the IL-10 levels
       produced by lipopolysaccharide (LPS)-stimulated splenic and peritoneal
       exudate cells were higher in young NZB/W F1 mice compared to those in
       C57BL/6 mice. Our data suggest that dysregulation between TH1 and TH2
       cells may play an important role in the pathogenesis of autoimmunity in
       NZB/W F1 mice.
 DE    Age Factors  Animal  Autoimmunity/*IMMUNOLOGY  Cells, Cultured
       Cytokines/*BIOSYNTHESIS/IMMUNOLOGY  Female  Immunophenotyping  Mice
       Mice, Inbred C57BL  Mice, Inbred NZB  Spleen/IMMUNOLOGY  Support,
       Non-U.S. Gov't  Th1 Cells/*IMMUNOLOGY/METABOLISM  Th2
       Cells/*IMMUNOLOGY/METABOLISM  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).