Document 0170
 DOCN  M9610170
 TI    Expression and immunogenicity of an 18-residue epitope of HIV1 gp41
       inserted in the flagellar protein of a Salmonella live vaccine.
 DT    9601
 AU    Newton SM; Joys TM; Anderson SA; Kennedy RC; Hovi ME; Stocker BA;
       Department of Microbiology and Immunology, Stanford University; School
       of Medicine, CA 94305-5402.
 SO    Res Microbiol. 1995 Mar-Apr;146(3):203-16. Unique Identifier : AIDSLINE
       MED/96008859
 AB    A synthetic oligonucleotide specifying residues 735-752 of the product
       of the env gene of HIV1 IIIB was inserted by blunt-end ligation at
       restriction sites in the hypervariable, antigenically determinant region
       IV of two flagellin genes. Its integration, in frame and correct
       orientation, into gene fliC(d) in plasmid pLS408 allowed production of
       functional flagella when the plasmid was placed in a flagellin-negative
       aroA live-vaccine Salmonella dublin strain, SL5928. Bacteria thus made
       motile were immobilized and agglutinated by anti-(735-752 peptide)
       serum; expression was also shown by immunoelectron-microscopy and by
       Western blot of whole-cell lysates. Enzyme immunoassay (EIA) of sera of
       mice given three doses by intraperitoneal injection of the live-vaccine
       strain producing chimeric flagellin, or of concentrated flagella from
       it, showed production of antibody with affinity for the peptide, and in
       some sera, also for r-gp160. Pooled serum from mice given five i.p.
       doses of the live vaccine strain expressing the gp41 epitope at the
       surface of its flagellar filaments had higher titres of anti-peptide and
       anti-r-gp160 antibody and weak neutralizing activity on the IIIB isolate
       (90% neutralization at 1/100). The sera of nine mice given two doses of
       the live vaccine by the oral route had either no or only very low titres
       of antibody to flagellar antigen d; they were therefore not tested for
       anti-peptide activity.
 DE    Animal  Antibody Formation/*IMMUNOLOGY  AIDS Vaccines/IMMUNOLOGY
       Bacterial Vaccines/IMMUNOLOGY  Blotting, Western
       Epitopes/GENETICS/*IMMUNOLOGY  Flagella/ULTRASTRUCTURE
       Flagellin/GENETICS/*IMMUNOLOGY  HIV-1/GENETICS/*IMMUNOLOGY  In Vitro
       Membrane Glycoproteins/GENETICS/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Rabbits  Salmonella/CHEMISTRY/GENETICS/IMMUNOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't, P.H.S.
       Vaccines, Attenuated/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).