Document 0324
 DOCN  M9610324
 TI    Toxicology of cupric salts on honeybees. IV--Gluconate and sulfate
       action on hemolymph trehalose activity in vivo and in vitro.
 DT    9601
 AU    Nectoux M; Bounias M; Popeskovic D; INRA Phytopharmacy Department,
       University of Avignon, France.
 SO    J Biochem Toxicol. 1995 Apr;10(2):79-86. Unique Identifier : AIDSLINE
       MED/96035095
 AB    A biphasic increase of hemolymph glucose levels was observed following
       injection to bees of cupric gluconate or sulfate, both potent agents for
       the control of Varroa jacobsoni, a parasitic mite of hives. The
       simultaneous injection to bees of 0.3 microM BAYg5421 (an inhibitor of
       alpha-glucosidases) quenched the response, suggesting a direct effect of
       2 nmol/bee cupric ions on trehaloses' activity. One nanomol of injected
       cupric gluconate increased the trehalose (Tre) activity by 233% in crude
       hemolymph extracts at 1 mM trehalose concentration, and exhibited
       biphasic dose-related effects with a maximum 15% increase at 0.5 mM
       cupric ion and a stabilized 20% inhibition from 4 mM, regardless of the
       anionic moiety. Upon partial purification of the enzyme complex, two
       fractions (FI = 75% and FII = 25% of total activity) were isolated that
       exhibited, respectively, less and more marked positive cooperatively
       than crude extract. Form I showed almost no susceptibility to either
       cupric derivatives, which indicated form II as the most likely target,
       with 68% and 72% increases with 0.25 mM cupric sulfate and 0.5 mM cupric
       gluconate, in presence of 16 mM trehalose.
 DE    Animal  Bees/DRUG EFFECTS/*PHYSIOLOGY  Copper/*TOXICITY  Female
       Gluconates/*TOXICITY  Hemolymph/METABOLISM  Hypoglycemic
       Agents/PHARMACOLOGY  Kinetics  Regression Analysis
       Trehalose/*METABOLISM  Trisaccharides/PHARMACOLOGY  JOURNAL ARTICLE

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