Document 0364
 DOCN  M9610364
 TI    CD4 down-modulation by ganglioside and phorbol ester inhibits human
       herpesvirus 7 infection.
 DT    9601
 AU    Yasukawa M; Inoue Y; Sada E; Yakushijin Y; Furukawa M; Fujita S; First
       Department of Internal Medicine, Ehime University School of; Medicine,
       Japan.
 SO    J Gen Virol. 1995 Sep;76 ( Pt 9):2381-5. Unique Identifier : AIDSLINE
       MED/96005065
 AB    Recently, data demonstrating that CD4 is an essential component of the
       receptor for human herpesvirus 7 (HHV-7) as well as for human
       immunodeficiency virus have been accumulating. Since gangliosides and
       phorbol esters are known to induce selective down-modulation of cell
       surface CD4 expression, it might be expected that treatment with these
       agents would interfere with HHV-7 infection of CD4+ T cells. The present
       study, undertaken to verify this possibility, demonstrated that addition
       of monosialoganglioside-GM1 or 12-O-tetradecanoylphorbol 13-acetate
       effectively induced disappearance of CD4 from the cell surface and also
       reduced HHV-7 infectivity, as judged by the CPE on virus-infected cells
       and studies of indirect immunofluorescence, TCID50 and semi-quantitative
       PCR of the HHV-7 genome. Taken together with previous studies, the
       present data strongly suggest that the CD4 molecule is a critical
       component of the receptor for HHV-7.
 DE    Antigens, CD4/*DRUG EFFECTS  Antigens, Viral/ANALYSIS  Antiviral
       Agents/*PHARMACOLOGY  Base Sequence  Cell Line  Cytopathogenic Effect,
       Viral  CD4-Positive T-Lymphocytes/CYTOLOGY  Down-Regulation (Physiology)
       DNA Primers  G(M1) Ganglioside/*PHARMACOLOGY  Herpesviridae
       Infections/VIROLOGY  Herpesvirus 7, Human/*DRUG EFFECTS/IMMUNOLOGY
       Human  Immunosuppressive Agents/*PHARMACOLOGY  Molecular Sequence Data
       Tetradecanoylphorbol Acetate/*PHARMACOLOGY  JOURNAL ARTICLE

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