Document 0368
 DOCN  M9610368
 TI    Analysis of cross reactivity of retrovirus proteases using a vaccinia
       virus-T7 RNA polymerase-based expression system.
 DT    9601
 AU    Luukkonen BG; Tan W; Fenyo EM; Schwartz S; Microbiology and Tumorbiology
       Center, Karolinska Institute,; Stockholm, Sweden.
 SO    J Gen Virol. 1995 Sep;76 ( Pt 9):2169-80. Unique Identifier : AIDSLINE
       MED/96005038
 AB    We have used the vaccinia virus-T7 RNA polymerase-based expression
       system for studies on the activity of proteases from various
       retroviruses on homologous and heterologous Gag polyproteins in
       eukaryotic cells. Proteases from human immunodeficiency virus (HIV)
       types 1 and 2, equine infectious anaemia virus, human T cell leukaemia
       virus type 1 and human spumavirus were produced and were shown to cleave
       their cognate Gag substrates produced in trans. Analysis of cross
       reactivity revealed that lentivirus proteases cleaved only lentivirus
       Gag proteins and oncovirus proteases acted primarily on oncovirus Gag
       proteins. The HIV-2 protease cleaved the HIV-1 Gag precursor almost as
       efficiently as HIV-1 protease. Expression of the 5' end of the human
       spumavirus pol gene revealed that it encodes a functional protease that
       acts specifically on the human spumavirus Gag polyprotein. This assay
       will allow further investigation on the activity and specificity of
       retrovirus proteases in eukaryotic cells.
 DE    Amino Acid Sequence  Animal  Base Sequence  Gene Products,
       gag/*METABOLISM  Gene Products, pol  Genetic Vectors  Hela Cells  Human
       HIV/ENZYMOLOGY/GENETICS  HTLV-I/ENZYMOLOGY/GENETICS  Infectious Anemia
       Virus, Equine/ENZYMOLOGY/GENETICS  Molecular Sequence Data  Peptide
       Peptidohydrolases/GENETICS/*METABOLISM  Recombinant Fusion
       Proteins/GENETICS/METABOLISM  Retroviridae/*ENZYMOLOGY/GENETICS  RNA
       Polymerases/GENETICS  Spumavirus/ENZYMOLOGY/GENETICS  Substrate
       Specificity  Support, Non-U.S. Gov't  Vaccinia Virus/*GENETICS  JOURNAL
       ARTICLE

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