Document 0380 DOCN M9610380 TI Microsporidium: modified technique for light microscopic diagnosis. DT 9601 AU Punpoowong B; Pitisuttithum P; Chindanond D; Phiboonnakit D; Leelasuphasri S; Department of Tropical Pathology, Hospital for Tropical Diseases,; Faculty of Tropical Medicine, Mahidol University, Bangkok,; Thailand. SO J Med Assoc Thai. 1995 May;78(5):251-4. Unique Identifier : AIDSLINE MED/96007040 AB Microsporiodosis caused by Enterocytozoon bieneusi is one of the opportunistic infections in HIV positive patients with chronic diarrhea. The organism is difficult to diagnose because of its small size, previously the diagnosis of this infection relied on identification of the organism under electron microscope. Until recently, the spores of this organism in stool specimens could be seen under light microscope by using various staining techniques. In this study, the modified trichrome staining technique was used to identify microsporidia spores with characteristic red belt-like stripes. Less time and less reagent are required by this modified technique than the conventional method. DE Animal AIDS-Related Opportunistic Infections/*DIAGNOSIS Feces/PARASITOLOGY Human Microspora Infections/*DIAGNOSIS Microsporida/*ISOLATION & PURIF JOURNAL ARTICLE SOURCE: National Library of Medicine. NOTICE: This material may be protected by Copyright Law (Title 17, U.S.Code).