Document 0402
 DOCN  M9610402
 TI    A nonlethal rat parvovirus infection suppresses rat T lymphocyte
       effector functions.
 DT    9601
 AU    McKisic MD; Paturzo FX; Gaertner DJ; Jacoby RO; Smith AL; Section of
       Comparative Medicine, Yale School of Medicine, New; Haven, CT
       06520-8016, USA.
 SO    J Immunol. 1995 Oct 15;155(8):3979-86. Unique Identifier : AIDSLINE
       MED/96003439
 AB    Inoculation of the UMass strain of rat virus (RV-UMass) into adult
       immunocompetent rats results in a prolonged subclinical infection that
       is resolved in 4 to 8 wk. Co-labeling studies, using in situ
       hybridization (ISH) and immunohistochemistry (IHC), confirmed that
       RV-UMass was lymphocytotropic and capable of infecting CD4+ and CD8+ T
       cells as well as B cells. ISH studies also revealed that virus
       replication was restricted in unstimulated cells but was productive in
       concanavalin A-stimulated lymphocytes. A corollary of productive
       infection of lymphocytes was the suppression of lymphocyte functions.
       Although RV-UMass did not appear to induce phenotypic changes during the
       course of infection, cells from infected rats had diminished
       proliferation and cytolytic responses. Both peripheral and mesenteric
       lymph node cells exhibited only partial recovery of their proliferative
       and cytolytic capacities one month after infection. Furthermore,
       RV-UMass-infected tissue culture maintained alloreactive CD4+ T cells in
       vitro, and a nonlethal infection of this T cell line inhibited Ag- and
       IL-2-induced proliferation. Because parvoviruses are widespread among
       laboratory rodents, these findings emphasize the importance of
       identifying and excluding parvovirus infection in rodents and in
       cultures of rat T lymphocytes.
 DE    Animal  Cell Line  Cytotoxicity, Immunologic  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  CD8-Positive T-Lymphocytes/IMMUNOLOGY  Disease
       Susceptibility  *Immune Tolerance  Lymphocyte Transformation
       Parvoviridae Infections/*IMMUNOLOGY  Parvovirus/*IMMUNOLOGY  Phenotype
       Rats  Rats, Inbred Lew  Rats, Inbred WF  Species Specificity  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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