Document 0429
 DOCN  M9610429
 TI    No relationship between skin-infiltrating TH2-like cells and
       allergen-specific IgE response in atopic dermatitis.
 DT    9601
 AU    Virtanen T; Maggi E; Manetti R; Piccinni MP; Sampognaro S; Parronchi P;
       De Carli M; Zuccati G; Romognani S; Department of Clinical Microbiology,
       University of Kuopio,; Finland.
 SO    J Allergy Clin Immunol. 1995 Sep;96(3):411-20. Unique Identifier :
       AIDSLINE MED/96013057
 AB    More than 500 CD4+ T-cell clones (TCCs) derived from the skin of eight
       patients with atopic dermatitis (AD), two patients with nonatopic
       dermatologic disorders, two patients with allergic rhinitis, and one
       healthy nonatopic donor were analyzed for both their pattern of cytokine
       production and their antigen specificity. The proportions of TCCs from
       patients with AD producing interleukin-4 in response to stimulation with
       phorbol 12-myristate 13-acetate plus anti-CD3 antibody were higher,
       whereas the proportions of interferon-gamma--producing TCCs were lower
       than those of control subjects. In two patients with AD, the majority of
       TCCs had a TH2/TH0-like phenotype, whereas in six patients with AD a
       TH1/TH0-like phenotype was prevalent. TCCs with a TH2/TH0-like phenotype
       were also isolated from the healthy skin of two patients with allergic
       rhinitis and one nonatopic donor. In contrast, no TH2-like TCCs were
       derived from the skin of the two patients with dermatologic disorders of
       nonallergic origin. No unambiguous correlations was found between the
       proportions of TCCs producing interleukin-4 or interferon-gamma (or of
       TCCs with TH2- or TH1-like profile) and the level of total serum IgE,
       suggesting that CD4+ T cells infiltrating the atopic skin do not play a
       major role in the production of serum IgE antibodies. When TCCs from
       five patients with AD were examined for their specificity, the
       proportions of allergen-specific (Dermatophagoides pteronyssinus and Lol
       p 1) clones were consistently 6% or lower even in patients with high
       titers of ryegrassor D. pteronyssinus-specific IgE antibodies. Because
       similar percentages of allergen-specific TCCs were found in skin from
       two healthy control subjects, the role of aeroallergens in favoring and
       maintaining skin lesions in patients with AD remains unclear.
 DE    Adolescence  Adult  Allergens/*IMMUNOLOGY  Clone Cells  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY/PATHOLOGY  Dermatitis,
       Atopic/*IMMUNOLOGY/*PATHOLOGY  Epitopes  Female  Human  IgE/*IMMUNOLOGY
       Interferon Type II/BIOSYNTHESIS  Interleukin-4/BIOSYNTHESIS  Lymphocyte
       Transformation  Male  Middle Age  Phenotype  Skin/*PATHOLOGY  Support,
       Non-U.S. Gov't  Th2 Cells/*IMMUNOLOGY/PATHOLOGY  JOURNAL ARTICLE

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