Document 0429 DOCN M9610429 TI No relationship between skin-infiltrating TH2-like cells and allergen-specific IgE response in atopic dermatitis. DT 9601 AU Virtanen T; Maggi E; Manetti R; Piccinni MP; Sampognaro S; Parronchi P; De Carli M; Zuccati G; Romognani S; Department of Clinical Microbiology, University of Kuopio,; Finland. SO J Allergy Clin Immunol. 1995 Sep;96(3):411-20. Unique Identifier : AIDSLINE MED/96013057 AB More than 500 CD4+ T-cell clones (TCCs) derived from the skin of eight patients with atopic dermatitis (AD), two patients with nonatopic dermatologic disorders, two patients with allergic rhinitis, and one healthy nonatopic donor were analyzed for both their pattern of cytokine production and their antigen specificity. The proportions of TCCs from patients with AD producing interleukin-4 in response to stimulation with phorbol 12-myristate 13-acetate plus anti-CD3 antibody were higher, whereas the proportions of interferon-gamma--producing TCCs were lower than those of control subjects. In two patients with AD, the majority of TCCs had a TH2/TH0-like phenotype, whereas in six patients with AD a TH1/TH0-like phenotype was prevalent. TCCs with a TH2/TH0-like phenotype were also isolated from the healthy skin of two patients with allergic rhinitis and one nonatopic donor. In contrast, no TH2-like TCCs were derived from the skin of the two patients with dermatologic disorders of nonallergic origin. No unambiguous correlations was found between the proportions of TCCs producing interleukin-4 or interferon-gamma (or of TCCs with TH2- or TH1-like profile) and the level of total serum IgE, suggesting that CD4+ T cells infiltrating the atopic skin do not play a major role in the production of serum IgE antibodies. When TCCs from five patients with AD were examined for their specificity, the proportions of allergen-specific (Dermatophagoides pteronyssinus and Lol p 1) clones were consistently 6% or lower even in patients with high titers of ryegrassor D. pteronyssinus-specific IgE antibodies. Because similar percentages of allergen-specific TCCs were found in skin from two healthy control subjects, the role of aeroallergens in favoring and maintaining skin lesions in patients with AD remains unclear. DE Adolescence Adult Allergens/*IMMUNOLOGY Clone Cells CD4-Positive T-Lymphocytes/IMMUNOLOGY/PATHOLOGY Dermatitis, Atopic/*IMMUNOLOGY/*PATHOLOGY Epitopes Female Human IgE/*IMMUNOLOGY Interferon Type II/BIOSYNTHESIS Interleukin-4/BIOSYNTHESIS Lymphocyte Transformation Male Middle Age Phenotype Skin/*PATHOLOGY Support, Non-U.S. Gov't Th2 Cells/*IMMUNOLOGY/PATHOLOGY JOURNAL ARTICLE SOURCE: National Library of Medicine. NOTICE: This material may be protected by Copyright Law (Title 17, U.S.Code).