Document 0520
 DOCN  M9610520
 TI    Novel glycolipids of Mycobacterium avium and related M. paratuberculosis
       strains of relevance to AIDS and Crohn's disease.
 DT    9601
 AU    Ohene-Gyan KA; Haagsma J; Davies MJ; Hounsell EF; Department of
       Bacteriology, Central Veterinary Institute,; Lelystad, The Netherlands.
 SO    Comp Immunol Microbiol Infect Dis. 1995 Jun;18(3):161-70. Unique
       Identifier : AIDSLINE MED/96002248
 AB    The polar glycolipid fractions of several mycobacterial strains of the
       closely related species M. avium and M. paratuberculosis have been
       analysed by thin layer chromatography (TLC), high pH anion exchange
       chromatography (HPAEC), gas-liquid chromatography (GC) and nuclear
       magnetic resonance (NMR) spectroscopy. The upper phase of a Folch
       partitioning (rather than the lower phase analysed by others) was
       subjected to TLC in solvent system chloroform-methanol-water 50:40:10
       v/v/v. A major band was purified from each mycobacterial strain.
       Monosaccharide analysis of that from M. avium A14 (from an AIDS patient)
       contained Glc, Ara, Man, Gal in ratios 7:4:3:2. whereas one strain of M.
       paratuberculosis (316F) had low levels of Ara, Gal and Man with major
       monosaccharides being Glc and two unidentified monosaccharides. A second
       M. paratuberculosis strain (J10) had a single TLC band containing only
       Glc. These known strains were compared to two slow growing mycobacterial
       isolates, one from a Crohn's patient and one isolated from armadillo.
       These were similar to J10 in only having Glc present: the former also
       had a similar NMR spectrum to J10, whereas the latter had a different
       NMR spectrum from any of the other strains analysed. The results
       therefore indicate that M. paratuberculosis strain 316F is more closely
       related to M. avium (from an AIDS patient) than it is to the classical
       M. paratuberculosis strain J10 and a Crohn's isolate.
 DE    AIDS-Related Opportunistic Infections/MICROBIOLOGY  Chromatography, Gas
       Chromatography, Ion Exchange  Chromatography, Thin Layer  Crohn
       Disease/COMPLICATIONS/MICROBIOLOGY  Glycolipids/*CHEMISTRY  Human
       Monosaccharides/ANALYSIS  Mycobacterium avium
       Complex/*CHEMISTRY/CLASSIFICATION/ISOLATION &  PURIF  Mycobacterium
       avium-intracellulare Infection/COMPLICATIONS/  MICROBIOLOGY
       Mycobacterium paratuberculosis/*CHEMISTRY/CLASSIFICATION/  ISOLATION &
       PURIF  Nuclear Magnetic Resonance
       Paratuberculosis/COMPLICATIONS/MICROBIOLOGY  Species Specificity
       JOURNAL ARTICLE

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