Document 0663
 DOCN  M9610663
 TI    Novel assay system favorable for the study of cell-to-cell transmission
       of HIV-1 and its application to the evaluation of anti-HIV drugs.
 DT    9601
 AU    Inouye Y; Kanamori T; Fujimoto Y; Sugiyama M; Yoshida T; Institute of
       Pharmaceutical Sciences, Hiroshima University School; of Medicine,
       Japan.
 SO    Biol Pharm Bull. 1995 Jun;18(6):920-2. Unique Identifier : AIDSLINE
       MED/96044634
 AB    The cell-to-cell transmission of human immunodeficiency virus type 1
       (HIV-1) was studied using MOLT-4 cells chronically infected with a
       variant strain of HIV-1SF-2 (MOLT-4/HIV-1SF-2H) and CD4+ human lymphoid
       MT-4 cells. MOLT-4/HIV-1SF-2H cells produced less than 1 TCID50
       infectious particles per day as determined by the cytopathogenicity in
       MT-4 cells. However, the expression of envelope glycoproteins gp120 and
       gp41 on the MOLT-4/HIV-1SF-2H cell membrane was satisfactory for
       syncytium formation with the uninfected MOLT-4 cells. When
       MOLT-4/HIV-1SF-2H and MT-4 cells were co-cultured, severe
       cytopathogenicity was observed in MT-4 cells without being accompanied
       by the formation of multi-nucleated cells. Thus, the system consisting
       of MOLT-4/HIV-1SF-2H and MT-4 cells is convenient for exclusive study of
       the mechanism of cell-to-cell transmission of HIV-1. Using various
       compounds, it was confirmed that cell-to-cell transmission required both
       gp120/gp41-CD4 binding and de novo DNA synthesis.
 DE    Acquired Immunodeficiency Syndrome/DRUG THERAPY/*VIROLOGY  Antiviral
       Agents/*PHARMACOLOGY  Cell Line  Cell-Free System  Cytopathogenic
       Effect, Viral  Drug Screening  Human  HIV-1/*DRUG EFFECTS
       Polymers/PHARMACOLOGY  Silicon Compounds/PHARMACOLOGY  Tungsten
       Compounds/PHARMACOLOGY  Ultraviolet Rays  JOURNAL ARTICLE

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