Document 0705
 DOCN  M9610705
 TI    Quantification of feline immunodeficiency virus (FIV) proviral DNA in
       peripheral blood mononuclear cells of cats infected with Japanese
       strains of FIV.
 DT    9601
 AU    Inoshima Y; Tomonaga K; Ikeda Y; Miyazawa T; Mikami T; Department of
       Veterinary Microbiology, Faculty of Agriculture,; University of Tokyo,
       Japan.
 SO    J Vet Med Sci. 1995 Jun;57(3):487-92. Unique Identifier : AIDSLINE
       MED/96031673
 AB    The polymerase chain reaction (PCR) method was applied for measurement
       of the proviral DNA copy number of feline immunodeficiency virus (FIV)
       in peripheral blood mononuclear cells (PBMC) of cats experimentally and
       naturally infected with FIV. In experimentally infected cats except one
       cat infected with the Petaluma strain, FIV-specific DNAs were
       efficiently amplified with the PCR method under the conditions used in
       this study. In the naturally FIV-infected cats, the specific DNAs were
       also amplified. We established a quantitative method for measurement of
       proviral DNA copy number in PBMC from cats infected with TM2-type of FIV
       strains, and found that the number was variable among the six cats
       examined, ranging from 10(4.0) to 10(5.7) copies per 10(5) PBMCs. This
       method can be applicable to cats naturally infected with FIV of
       TM2-type. Proviral DNA quantitation developed here could be useful as an
       additional parameter to evaluate the relationships among the proviral
       load, immune response and development of the clinical symptoms, and to
       monitor efficacy of antiviral therapy in vivo.
 DE    Animal  Antibodies, Viral/*BLOOD  Base Sequence  *Cat Diseases  Cats
       Comparative Study  CD4-CD8 Ratio  DNA Primers  DNA, Viral/*BLOOD
       Enzyme-Linked Immunosorbent Assay/METHODS  Feline Acquired
       Immunodeficiency Syndrome/*DIAGNOSIS/IMMUNOLOGY/  VIROLOGY
       Gamma-Globulins/ANALYSIS  Immunodeficiency Virus, Feline/*ISOLATION &
       PURIF  Lymphocytes/*VIROLOGY  Molecular Sequence Data  Polymerase Chain
       Reaction/METHODS/VETERINARY  Proviruses/*ISOLATION & PURIF  Sensitivity
       and Specificity  JOURNAL ARTICLE

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