Document 0761
 DOCN  M9610761
 TI    Early cytokine production in pulmonary Cryptococcus neoformans
       infections distinguishes susceptible and resistant mice.
 DT    9601
 AU    Hoag KA; Street NE; Huffnagle GB; Lipscomb MF; Department of
       Microbiology, University of Texas Southwestern; Medical Center, Dallas
       75235-8576, USA.
 SO    Am J Respir Cell Mol Biol. 1995 Oct;13(4):487-95. Unique Identifier :
       AIDSLINE MED/96025070
 AB    A murine pulmonary infection model utilizing intratracheal inoculation
       of Cryptococcus neoformans was used to analyze cytokines produced in
       response to opportunistic pathogens acquired via the respiratory tract.
       The specific question asked was whether early cytokine secretion in
       lung-associated lymph nodes (LALN) would predict whether this organism
       would be cleared from the lung. Lung colony-forming units (CFU) were
       analyzed in two strains of mice over 12 wk, and lung clearance was found
       to be strain dependent. C.B-17 mice reduced their lung CFU burden
       between day 7 and day 14 of infection, had significantly higher in lung
       CFU than C.B-17 mice. The capacity of cells from lungs and LALN to
       secrete cytokines was significantly different between the strains when
       assessed at day 7 and day 14 after inoculation. When compared with
       sensitive C57BL/6 mice 7 days after infection, resistant C.B-17 mice
       demonstrated (1) increased interferon-gamma secretion by LALN cells in
       vitro in response to media alone, heat-killed cryptococci, and the T
       cell mitogen concanavalin A and (2) increased interleukin (IL)-2
       secretion by both LALN and lung cells in response to concanavalin A.
       IL-4 and IL-10 were comparable or undetectable in both mouse strains,
       whereas IL-5 was significantly higher in all lung cell cultures of
       C57BL/6 mice. Thus, an early regional Th1 immune response in C.B-17 mice
       correlated with resistance to the organism, whereas the absence of this
       response in C57BL/6 mice correlated with susceptibility.
 DE    Animal  Antigens, Fungal/IMMUNOLOGY  Cells, Cultured  Comparative Study
       Concanavalin A/PHARMACOLOGY  Cryptococcosis/*IMMUNOLOGY/MICROBIOLOGY
       Cryptococcus/GROWTH & DEVELOPMENT/*IMMUNOLOGY  CD4 Lymphocyte Count
       Disease Susceptibility  Female  Immunity, Natural  Interferon Type
       II/*BIOSYNTHESIS  Interleukins/*BIOSYNTHESIS
       Lung/*IMMUNOLOGY/MICROBIOLOGY  Lung Diseases,
       Fungal/*IMMUNOLOGY/MICROBIOLOGY  Lymph Nodes/IMMUNOLOGY  Lymphocyte
       Transformation  Mice  Mice, Inbred BALB C  Mice, Inbred C57BL  Species
       Specificity  Spleen/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).