Document 0006
 DOCN  M9620006
 TI    Receptor-linked antigen delivery system. Importance of autologous alpha
       2-macroglobulin in the development of peptide vaccine.
 DT    9602
 AU    Mitsuda S; Nakagawa T; Nakazato H; Ikai A; Department of Biological
       Sciences, Faculty of Bioscience and; Biotechnology, Tokyo Institute of
       Technology, Yokohama, Japan.
 SO    Biochem Biophys Res Commun. 1995 Nov 2;216(1):399-405. Unique Identifier
       : AIDSLINE MED/96067578
 AB    We have hijacked a process of the receptor-mediated endocytosis to
       transport peptide antigens into antigen presenting cells (APCs) for the
       purpose of increasing the level of antigen presentation (named
       Receptor-Linked Antigen Delivery System (R-LADS)). By coupling an
       endogenous plasma proteinase inhibitor alpha 2-macroglobulin (alpha 2M)
       to a synthetic peptide having a partial sequence of HIV-1 envelope
       protein, alpha 2M was made to carry the peptide into APCs as a part of
       the normal alpha 2M cycle, which resulted in an increased production of
       specific antibodies against the peptide (Mitsuda, S., Nakagawa, T.,
       Osada, T., Shimamoto, T., Nakazato, H. and Ikai, A. (1993) Biochem.
       Biophys. Res. Commun. 194, 1155-1160). We demonstrate here that this
       procedure becomes a more efficient tool for antibody production when
       autologous transporter protein was used. By using murine alpha 2M (m
       alpha 2M) instead of heterologous human alpha 2M (h alpha 2M) when mice
       were experimental animals, we were able to dramatically enhance the
       production level of anti-HIV-1 peptide antibodies and shorten the period
       which is needed for antibody production. We aim to develop effective
       peptide vaccines by further improving this system.
 DE    alpha-Macroglobulins/*IMMUNOLOGY  Animal  Antigen-Presenting
       Cells/*IMMUNOLOGY/METABOLISM  AIDS Vaccines/*ADMINISTRATION & DOSAGE
       Electrophoresis, Polyacrylamide Gel  *Endocytosis  Female  Gene
       Products, env/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  Human  HIV
       Antibodies/*BIOSYNTHESIS  HIV-1/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Receptors, Immunologic/*PHYSIOLOGY  *Vaccines  Vaccines,
       Synthetic/*ADMINISTRATION & DOSAGE  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).