Document 0046
 DOCN  M9620046
 TI    In vivo proviral burden and viral RNA expression in T cell subsets of
       patients with human T lymphotropic virus type-1-associated
       myelopathy/tropical spastic paraparesis.
 DT    9602
 AU    Cho I; Sugimoto M; Mita S; Tokunaga M; Imamura F; Ando M; First
       Department of Internal Medicine, Kumamoto University School; of
       Medicine, Japan.
 SO    Am J Trop Med Hyg. 1995 Oct;53(4):412-8. Unique Identifier : AIDSLINE
       MED/96074151
 AB    We used in situ hybridization combined with immunocytochemistry, cell
       sorting, and the polymerase chain reaction (PCR) to investigate clinical
       events in three asymptomatic carriers of human T lymphotrophic virus
       type-1 (HTLV-1) and ten patients with HTLV-1-associated
       myelopathy/tropical spastic paraparesis (HAM/TSP). The objective was to
       determine which T cell subset of peripheral blood mononuclear cells
       (PBMC), CD4 or CD8, were infected by HTLV-1 and the manner in which
       HTLV-1 proviral DNA was expressed at the level of the single cell. Both
       CD4-positive and CD8-positive cells of the PBMC from five patients with
       HAM/TSP were infected with HTLV-1. The proportion of HTLV-1-infected
       cells was 2.5-40% in the CD4-positive subset and 1.0-65% in the CD8-
       positive subset, when quantified by PCR using HTLV-1-infected MT2 cells
       as a positive standard. Proviral DNA of HTLV-1 was expressed in both
       CD4-positive cells and CD8-positive cells of the PBMC from six patients
       with HAM/TSP and three asymptomatic HTLV-1 carriers. In patients with
       HAM/TSP, the proportion of the cells expressing HTLV-1 proviral DNA was
       0.02-0.1% in both subsets. In asymptomatic carriers, the expression of
       HTLV-1 proviral DNA was 0.01-0.02% in the CD4-positive subset and 0.01%
       in the CD8-positive subset. Therefore, HTLV-1 possessed similar in vivo
       cellular tropism for both CD4-positive cells and CD8-positive cells and
       HTLV-1 proviral DNA was expressed in vivo in both circulating T cell
       subsets.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Adult  Aged  Aged, 80 and over  Base Sequence  Cell Separation  DNA
       Primers/CHEMISTRY  DNA, Viral/BIOSYNTHESIS/CHEMISTRY  Female  Flow
       Cytometry  Gene Expression Regulation, Viral  Human
       HTLV-I/GENETICS/*PHYSIOLOGY  Immunohistochemistry  In Situ Hybridization
       Male  Microspheres  Middle Age  Molecular Sequence Data  Paraparesis,
       Tropical Spastic/*VIROLOGY  Polymerase Chain Reaction
       Proviruses/GENETICS/*PHYSIOLOGY  RNA, Messenger/BIOSYNTHESIS  RNA,
       Viral/*BIOSYNTHESIS  T-Lymphocyte Subsets/*VIROLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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