Document 0066
 DOCN  M9620066
 TI    Enhanced proliferation and IL-2 secretion by lung lymphocytes from
       HIV-infected subjects.
 DT    9602
 AU    Spain BA; Soliman DM; Sidner RA; Twigg HL; Department of Medicine,
       Indiana University Medical Center,; Indianapolis 46202, USA.
 SO    Am J Physiol. 1995 Oct;269(4 Pt 1):L498-506. Unique Identifier :
       AIDSLINE MED/96043434
 AB    Human immunodeficiency virus (HIV)-positive patients frequently develop
       a CD3+/CD8+ cytotoxic T cell lymphocytic alveolitis. This could occur
       through in situ expansion of lung lymphocytes. We evaluated lung and
       blood lymphocyte proliferation in asymptomatic HIV-infected individuals
       by measuring spontaneous and cytokine-induced tritiated thymidine
       incorporation. Interleukin (IL)-2 and IL-4 secretion was determined with
       the use of enzyme-linked immunosorbent assay, Western blotting, and
       immunoprecipitation techniques. Spontaneous proliferation by lung
       lymphocytes from HIV-positive patients was significantly greater than
       that of normal volunteers. Proliferation was confined to the CD8+
       lymphocyte subset. Over time, spontaneous proliferation declined unless
       autologous alveolar macrophages (AM) were added, suggesting AM were
       providing additional stimulatory signals to lung lymphocytes. Lung and
       blood lymphocytes proliferated in response to IL-2 but not IL-4.
       Lymphocytes in HIV-infected lung spontaneously produced and secreted
       more IL-2 than either normal lung lymphocytes or autologous blood
       lymphocytes. IL-4 production was not detectable in either group. These
       findings support the hypothesis that lymphocytic alveolitis in
       asymptomatic HIV-positive patients results from IL-2-dependent in situ
       proliferation of CD3+/CD8+ cytotoxic T cells.
 DE    Adult  Blood Cells/PATHOLOGY  Cell Division/DRUG EFFECTS  Human  HIV
       Infections/*METABOLISM/*PATHOLOGY
       Interleukin-2/BIOSYNTHESIS/PHARMACOLOGY/*SECRETION
       Interleukin-4/BIOSYNTHESIS/PHARMACOLOGY  Lung/METABOLISM/*PATHOLOGY
       Lymphocytes/*PATHOLOGY/*SECRETION  Support, U.S. Gov't, P.H.S.  JOURNAL
       ARTICLE

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