Document 0093
 DOCN  M9620093
 TI    Specificity of intrathecal IgG synthesis for HTLV-1 core and envelope
       proteins in HAM/TSP.
 DT    9602
 AU    Kitze B; Puccioni-Sohler M; Schaffner J; Rieckmann P; Weber T;
       Felgenhauer K; Bodemer W; Department of Neurology, University of
       Gottingen, Germany.
 SO    Acta Neurol Scand. 1995 Sep;92(3):213-7. Unique Identifier : AIDSLINE
       MED/96050969
 AB    INTRODUCTION--In patients with human T-cell lymphotropic virus type 1
       (HTLV-1) associated myelopathy/tropical spastic paraparesis (HAM/TSP),
       we investigated the significance of HTLV-1 specific antibodies in the
       cerebrospinal fluid (CSF). MATERIAL AND METHODS--The quantities of
       HTLV-1 specific immunoglobulin G (IgG) in paired CSF and serum were
       evaluated by a sensitive enzyme immunoassay (EIA). The specificity of
       antiviral IgG was determined by radioimmunoprecipitation of HTLV-1
       antigens. RESULTS--In 17 of 20 HAM/TSP patients, quantitative evaluation
       by EIA supplied evidence for antiviral IgG synthesis within the CNS.
       Radioimmunoprecipitation demonstrated IgG antibodies against HTLV-1
       envelope and core proteins in all HAM/TSP CSF and sera tested. Regarding
       the 3 sample pairs indeterminate in EIA for intrathecal synthesis, 2
       showed stronger precipitation of HTLV-1 antigens by CSF IgG than by
       equal amounts of serum IgG. CONCLUSION--Intrathecal antibody synthesis
       specific for both HTLV-1 core and envelope antigens is common in
       HAM/TSP, thus providing conclusive evidence for an immune response to
       HTLV-1 within the CNS.
 DE    Antibody Specificity/*IMMUNOLOGY  Blood-Brain Barrier/IMMUNOLOGY  Brazil
       Human  HTLV-I Antibodies/*CEREBROSPINAL FLUID  HTLV-I
       Antigens/*IMMUNOLOGY  IgG/*CEREBROSPINAL FLUID  Immunoenzyme Techniques
       Iran  Paraparesis, Tropical Spastic/DIAGNOSIS/*IMMUNOLOGY
       Radioimmunoprecipitation Assay  Viral Core Proteins/*IMMUNOLOGY  Viral
       Envelope Proteins/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).