Document 0116
 DOCN  M9620116
 TI    Quantitative PCR as a method for monitoring retroviral infection on the
       gene level.
 DT    9602
 AU    Yolov AA; Kozlova AV; Yaroslavtseva NG; Mednikov BM; Karamov EV; D.I.
       Ivanovski Institute of Virology, Moscow, Russia.
 SO    Virus Genes. 1995;10(1):45-51. Unique Identifier : AIDSLINE
       GENBANK/M15390
 AB    For monitoring retroviral infection on the gene level, we propose the
       use of quantitative PCR with two internal standards: one for a fragment
       of the viral genome and the other for the host cell marker gene. The
       standards (one for HIV and the other for a human DNA marker gene HLA-DQ
       alpha) were constructed by PCR-mediated joining of DNA fragments and
       were found to be effective in quantitative PCR despite rather different
       structures of amplified fragments in target and standard DNAs. The
       number of HIV DNA copies was found to be 2-500 per 1000 lymphocytes in
       blood from HIV-infected patients and up to 5000+ per 1000 cells in
       chronically infected cell lines. The degree of infection thus measured
       was found to change over the course of treatment.
 DE    Base Sequence  Cell Line  DNA Primers  DNA, Viral  Human  HIV
       Infections/BLOOD/*VIROLOGY  HIV-1/*GENETICS  HLA-DQ Antigens/*GENETICS
       Lymphocytes/VIROLOGY  Molecular Sequence Data  *Polymerase Chain
       Reaction  JOURNAL ARTICLE

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