Document 0200
 DOCN  M9620200
 TI    Residual expression of reporter genes in constructs mimicking HIV genome
       organization.
 DT    9602
 AU    Chenciner N; Pedroza Martins L; Hanania N; Stratford Perricaudet L;
       Perricaudet M; Wain-Hobson S; Unite de Retrovirologie moleculaire,
       Institut Pasteur, Paris,; France.
 SO    Res Virol. 1995 May-Jun;146(3):171-8. Unique Identifier : AIDSLINE
       MED/96075767
 AB    Plasmids were constructed whereby the expression of a reporter gene,
       either the cDNA corresponding to the secreted form of human alkaline
       phosphatase (SEAP) or the herpes simplex virus type 1 (HSV1) thymidine
       kinase (tk) gene, was rendered dependent upon the expression of the
       human immunodeficiency virus type 1 (HIV1) tat and rev proteins. The
       SEAP or tk genes were placed between HIV1 splice donor and acceptor
       sites. One SEAP construct carried a series of alternating splice donor
       and acceptor sites. In all cases, the rev response element mapped within
       an intron. Despite such mimicry of the HIV1 genome, residual expression
       of the reporter gene in the absence of tat and rev was observed. These
       results, as well as non-specific T-cell recruitment, suggest limits to
       the specificity of using HIV-activated toxic gene expression to kill
       HIV-infected cells.
 DE    Acquired Immunodeficiency Syndrome/THERAPY/VIROLOGY  Alkaline
       Phosphatase/GENETICS  Animal  Base Sequence  Cell Line  DNA Primers
       Gene Expression Regulation, Enzymologic  Gene Expression Regulation,
       Viral  Gene Products, rev/GENETICS  Gene Products, tat/GENETICS  Gene
       Therapy  *Genes, Reporter  Genetic Vectors  Genome, Viral  Herpesvirus
       1, Human/ENZYMOLOGY/GENETICS  Human  HIV-1/*GENETICS  Mice  Molecular
       Sequence Data  RNA, Messenger  Support, Non-U.S. Gov't
       T-Lymphocytes/VIROLOGY  Thymidine Kinase/GENETICS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).