Document 0253
 DOCN  M9620253
 TI    Cleavage of double-stranded DNA by
       'metalloporphyrin-linker-oligonucleotide' molecules: influence of the
       linker.
 DT    9602
 AU    Bigey P; Pratviel G; Meunier B; Laboratoire de Chimie de Coordination du
       CNRS, Toulouse, France.
 SO    Nucleic Acids Res. 1995 Oct 11;23(19):3894-900. Unique Identifier :
       AIDSLINE MED/96038845
 AB    Manganese porphyrin-linker-triple-helix-forming oligonucleotide
       molecules were prepared and their ability to cleave in vitro a
       double-stranded DNA target present in the HIV-1 genome was studied. The
       nature of the linker is a determining factor of the cleavage efficiency.
       Cleavage yields as high as 80% were observed when the linker was a
       spermine residue and in the absence of a large excess of free spermine
       known to stabilize triplex structures. The hydrophobic nature of
       aliphatic diamine linker modified the cleaver-DNA interactions and
       reduced the efficiency of DNA cleavage.
 DE    Antiviral Agents  Base Sequence  Cations, Divalent  Drug Stability  *DNA
       Damage  DNA, Viral/*METABOLISM  Heat  HIV-1/*GENETICS
       Magnesium/PHARMACOLOGY  Metalloporphyrins/CHEMISTRY/*METABOLISM
       Molecular Sequence Data  Molecular Structure
       Oligonucleotides/CHEMISTRY/*METABOLISM  Sodium/PHARMACOLOGY
       Spermine/CHEMISTRY  Structure-Activity Relationship  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).