Document 0386
 DOCN  M9620386
 TI    Frequent disruption of the Nf1 gene by a novel murine AIDS virus-related
       provirus in BXH-2 murine myeloid lymphomas.
 DT    9602
 AU    Cho BC; Shaughnessy JD Jr; Largaespada DA; Bedigian HG; Buchberg AM;
       Jenkins NA; Copeland NG; Mammalian Genetics Laboratory, ABL-Basic
       Research Program,; NCI-Frederick Cancer Research and Development Center,
       Maryland; 21702, USA.
 SO    J Virol. 1995 Nov;69(11):7138-46. Unique Identifier : AIDSLINE
       MED/96013817
 AB    Evi-2, a common site of viral integration in BXH-2 myeloid lymphomas, is
       located within a large intron of the Nf1 tumor suppressor gene. Viral
       integration at Evi-2 appears to induce disease by disrupting normal Nf1
       expression. During our attempts to characterize the nature of the
       proviruses located at Evi-2, we found that approximately half of the
       proviruses were defective nonecotropic proviruses (A. M. Buchberg, H. G.
       Bedigian, N. A. Jenkins, and N. G. Copeland, Mol. Cell. Biol.
       10:4658-4666, 1990). This was surprising, since most proviruses
       characterized at other BXH-2 common integration sites are full-length
       ecotropic viruses. In the studies described here, we found that this
       defective provirus carries two large deletions, one in pol and one in
       env, and is structurally related to another murine retrovirus, the
       murine AIDS retrovirus. By using oligonucleotide probes specific for
       this defective provirus, designated MRV, we showed that MRV-related
       proviruses are carried as endogenous germ line proviruses in most inbred
       strains. In addition, we identified the endogenous MRV provirus that
       gives rise to the defective proviruses identified at Evi-2. We present a
       model that accounts for the positive selection of MRV proviruses at
       Evi-2, which may allow selective identification of common viral
       integration sites harboring tumor suppressor genes.
 DE    Acquired Immunodeficiency Syndrome/GENETICS  Amino Acid Sequence  Animal
       Base Sequence  Blotting, Southern  *Chromosome Mapping  Comparative
       Study  DNA Primers  DNA, Neoplasm/ANALYSIS/GENETICS  DNA,
       Viral/ANALYSIS/GENETICS  Gene Library  Gene Products,
       gag/CHEMISTRY/GENETICS  *Genes, gag  *Genes, Neurofibromatosis 1
       Lymphoma/*GENETICS/*VIROLOGY  Mice  Mice, Inbred Strains  Molecular
       Sequence Data  Murine Acquired Immunodeficiency Syndrome/*VIROLOGY
       Oligonucleotide Probes  Proviruses/*GENETICS/ISOLATION & PURIF
       Repetitive Sequences, Nucleic Acid  Retroviridae/*GENETICS/ISOLATION &
       PURIF  Sequence Homology, Amino Acid  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).