Document 0388
 DOCN  M9620388
 TI    Syncytium induction in primary CD4+ T-cell lines from normal donors by
       human immunodeficiency virus type 1 isolates with non-syncytium-inducing
       genotype and phenotype in MT-2 cells.
 DT    9602
 AU    Todd BJ; Kedar P; Pope JH; Sir Albert Sakzewski Virus Research Centre,
       Royal Children's; Hospital, Brisbane, Queensland, Australia.
 SO    J Virol. 1995 Nov;69(11):7099-105. Unique Identifier : AIDSLINE
       MED/96013812
 AB    Human immunodeficiency virus type 1 (HIV-1) isolates classified as
       syncytium-inducing (SI) or non-SI (NSI) in the MT-2 T-cell line exhibit
       characteristic sequence differences in the V1-V2 and V3 regions of the
       env gene. Seven HIV-1 isolates were phenotyped as NSI or SI in the MT-2
       cell line. Unexpectedly, all four NSI viruses induced large syncytia 4
       to 8 days postinoculation in a panel of five primary CD4+ T-cell lines
       (including two clones) generated from the peripheral blood of normal
       donors by exposure to infectious HIV-1, inactivated HIV-1, or
       Epstein-Barr virus. The primary T-cell lines yielded neither HIV-1
       provirus nor infectious HIV by PCR analysis or exhaustive coculture with
       phytohemagglutinin-treated blast cells. Three isolates (TC354, PK1, and
       PK2) were biologically cloned and retained their SI or NSI phenotypes in
       MT-2 and primary T-cell lines. The biologically cloned provirus DNA was
       also used to clone and sequence the relevant V2 and V3 regions of the
       env genes. The amino acid sequences of the V2 and V3 regions were
       characteristic of patterns already reported for the NSI, switch NSI, and
       SI phenotypes, respectively. This evidence precludes the possibility
       that these results were due to contamination of the NSI isolates with SI
       virus. The results unequivocally indicate that HIV-1 isolates with the
       NSI genotype and phenotype in MT-2 cells may actively induce syncytia in
       cloned CD4+ T cells in vitro and support the view that direct cytopathic
       effects may contribute to the steady decline in CD4+ T cells in
       asymptomatic HIV-1-seropositive patients without detectable SI virus.
 DE    Amino Acid Sequence  Cell Line  Coculture  Comparative Study  Consensus
       Sequence  CD4-Positive T-Lymphocytes/*VIROLOGY  Flow Cytometry  Genotype
       *Giant Cells/CYTOLOGY/VIROLOGY  Herpesvirus 4, Human/PATHOGENICITY
       Human  HIV Envelope Protein gp120/CHEMISTRY/ISOLATION & PURIF  HIV
       Seronegativity/IMMUNOLOGY  HIV Seropositivity/IMMUNOLOGY/VIROLOGY
       HIV-1/GENETICS/ISOLATION & PURIF/*PHYSIOLOGY  Immunophenotyping
       Kinetics  Molecular Sequence Data  Phenotype  Polymerase Chain Reaction
       Reference Values  Support, Non-U.S. Gov't  *Virus Replication  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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