Document 0464
 DOCN  M9620464
 TI    Efficient replication of human immunodeficiency virus type 1 and measles
       virus in a human-to-mouse graft versus host disease model permits
       immunization research.
 DT    9602
 AU    Huppes W; Tenner-Racz K; Kraal G; Health Research-TNO, HV Rijswijk, The
       Netherlands.
 SO    J Gen Virol. 1995 Nov;76 ( Pt 11):2707-15. Unique Identifier : AIDSLINE
       MED/96068803
 AB    An acute graft versus host disease (GvHD) murine model was developed to
       study the pathogenic and protective mechanisms against viruses that
       replicate in cells of the human immune system. The model allowed
       efficient replication of lymphotropic, macrophage and amphitropic
       strains of human immunodeficiency virus type 1 (HIV-1) and measles virus
       (MV). Cytopathic lymphotropic strains of HIV-1 and a wild-type MV strain
       replicated in a 'burst'-like manner, whereas a non-cytopathic
       lymphotropic HIV-1 strain and all macrophage-tropic HIV-1 strains caused
       persistent infection of the graft. The replication kinetics of infection
       with these viruses were highly reproducible and were very similar to
       those observed in natural infection of humans. Infection with these
       viruses, with the exception of HIV-1SF2, led to a significant delay and
       abrogation of the GvHD, indicating a direct immunosuppressive effect.
       Interestingly, infection with the lymphotropic HIV-1SF2 strain was
       rapidly and spontaneously abrogated. The model was also shown to be
       suitable for the evaluation of passive immunization strategies.
       Administration of a combination of antibodies against the HIV-1 V3 loop
       and the HIV-1 CD4 binding sites prevented subsequent infection with
       HIV-1IIIB. In contrast, administration of CD4 binding site specific
       human monoclonal antibody at a concentration that would neutralize the
       virus in vitro enhanced in vivo infection with HIV-1IIIB. The model also
       allowed evaluation of in vivo immunization studies. Immunization with a
       live attenuated measles vaccine resulted in protection from a wild-type
       MV challenge, whereas immunization with a subunit candidate vaccine
       appeared to give partial protection.
 DE    Animal  Antigens, CD4/METABOLISM  Binding Sites  Cell Line  Disease
       Models, Animal  Graft vs Host Disease/IMMUNOLOGY/*VIROLOGY  Hela Cells
       Human  HIV Antibodies/IMMUNOLOGY  HIV Envelope Protein gp120/IMMUNOLOGY
       HIV-1/IMMUNOLOGY/*PHYSIOLOGY  Immunization  ISCOMs/IMMUNOLOGY  Measles
       Vaccine/IMMUNOLOGY  Measles Virus/IMMUNOLOGY/*PHYSIOLOGY  Mice  Mice,
       Inbred BALB C  Mice, Inbred CBA  Mice, SCID  Peptide
       Fragments/IMMUNOLOGY  Reproducibility of Results  Support, Non-U.S.
       Gov't  Transplantation, Heterologous  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).