Document 0504
 DOCN  M9620504
 TI    Identification of virulence-associated antigens and plasmids in
       Rhodococcus equi from patients with AIDS.
 DT    9602
 AU    Takai S; Imai Y; Fukunaga N; Uchida Y; Kamisawa K; Sasaki Y; Tsubaki S;
       Sekizaki T; Department of Animal Hygiene, School of Veterinary Medicine
       and; Animal Sciences, Kitasato University, Towada, Japan.
 SO    J Infect Dis. 1995 Nov;172(5):1306-11. Unique Identifier : AIDSLINE
       MED/96036388
 AB    Rhodococcus equi is an emerging opportunistic pathogen of human
       immunodeficiency virus-infected patients. However, little is known about
       the characteristics of R. equi isolates from humans. This study
       characterized the plasmid content, expression of a virulence-associated
       antigen, and mouse virulence of 19 R. equi isolates from patients with
       and without AIDS. EcoRI digestion patterns and Southern, Western, and
       virulence analyses of these isolates with cryptic plasmids allowed
       definition of a new category of R. equi. Isolates from patients with
       AIDS tended either to be virulent and have 15- to 17-kDa antigens and an
       85-kb plasmid (10(6) bacteria needed for lethality) or have intermediate
       virulence (10(7) bacteria needed for lethality) and one of four distinct
       large plasmids that share DNA homology and express a 20-kDa antigen.
       Most of the non-AIDS isolates were avirulent (> 10(8) bacteria needed
       for lethality) and did not express any of these antigens.
 DE    Acquired Immunodeficiency Syndrome/*MICROBIOLOGY  Actinomycetales
       Infections/COMPLICATIONS/*MICROBIOLOGY/VETERINARY  Animal  Antibodies,
       Monoclonal  AIDS-Related Opportunistic Infections/*MICROBIOLOGY
       Blotting, Southern  Blotting, Western  DNA Probes  Electrophoresis,
       Polyacrylamide Gel  Enzyme-Linked Immunosorbent Assay  Female  Horse
       Diseases  Horses  Human  Mice  Mice, Inbred BALB C/IMMUNOLOGY  *Plasmids
       Restriction Mapping  Rhodococcus equi/*GENETICS/ISOLATION &
       PURIF/*PATHOGENICITY  Support, Non-U.S. Gov't  Virulence  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).