Document 0516
 DOCN  M9620516
 TI    Comparison of p56lck and p59fyn protein expression in thymocyte subsets,
       peripheral T cells, NK cells, and lymphoid cell lines.
 DT    9602
 AU    Olszowy MW; Leuchtmann PL; Veillette A; Shaw AS; Center for Immunology,
       Washington University School of Medicine,; St. Louis, MO 63110, USA.
 SO    J Immunol. 1995 Nov 1;155(9):4236-40. Unique Identifier : AIDSLINE
       MED/96025880
 AB    The expression of the src-family kinases, p56lck and p59fyn, is critical
       for thymocyte development and TCR-mediated signal transduction, and may
       be important for signaling through other lymphoid receptors as well.
       Overexpression studies have demonstrated that the levels of p56lck and
       p59fyn expression can affect T cell development and signaling through
       the TCR. Therefore, it is likely that their exact expression levels play
       an important role in modulating signaling in thymocytes, mature T cells,
       and other lymphocytes. Here, we used quantitative immunoblotting to
       measure p56lck and p59fyn protein expression levels in thymocyte
       subsets, peripheral T cells, NK cells, and lymphoid cell lines. p59fyn
       expression levels were similar to p56lck in most cells that were
       examined demonstrating that p59fyn is abundantly expressed in T cells.
       In addition, we found that p56lck protein expression is equivalent in
       CD4 and CD8 double-negative, double-positive, and single-positive
       thymocytes. In contrast, p59fyn expression levels were significantly
       lower in double-positive thymocytes than in the other thymocyte
       subpopulations. Finally, we demonstrate that p56lck and p59fyn
       expression varies greatly in a number of cell lines used to study T cell
       activation and that IL-2 treatment can dynamically regulate p56lck and
       p59fyn expression in some cells.
 DE    src-Family Kinases/*BIOSYNTHESIS/IMMUNOLOGY  Animal  Cell Line
       Comparative Study  CD4-Positive T-Lymphocytes/METABOLISM  CD8-Positive
       T-Lymphocytes/METABOLISM  Female  Human  Interleukin-2/PHARMACOLOGY
       Killer Cells, Natural/DRUG EFFECTS/*METABOLISM  Mice  Mice, Inbred BALB
       C  Mice, SCID  Protein-Tyrosine Kinase/*BIOSYNTHESIS/IMMUNOLOGY
       Proto-Oncogene Proteins/*BIOSYNTHESIS/IMMUNOLOGY  Support, U.S. Gov't,
       P.H.S.  T-Lymphocyte Subsets/*METABOLISM  Tumor Cells, Cultured  JOURNAL
       ARTICLE

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