Document 0533
 DOCN  M9620533
 TI    Induction of cell-mediated immune responses to human immunodeficiency
       virus type 1 Gag protein by using Listeria monocytogenes as a live
       vaccine vector.
 DT    9602
 AU    Frankel FR; Hegde S; Lieberman J; Paterson Y; Department of
       Microbiology, University of Pennsylvania, School of; Medicine,
       Philadelphia 19104, USA.
 SO    J Immunol. 1995 Nov 15;155(10):4775-82. Unique Identifier : AIDSLINE
       MED/96062295
 AB    Cytolytic T cells, acting through cytokines or by direct lysis of
       infected target cells, have been shown to play a significant role in the
       control of viral infections and may be responsible for the prolonged
       asymptomatic phase following infection by HIV. Accordingly, methods that
       can generate strong cell-mediated immune responses may be useful in the
       development of prophylactic and therapeutic vaccines against HIV.
       Listeria monocytogenes is a Gram-positive intracellular microorganism
       that elicits strong cell-mediated immune responses against its own
       secreted proteins following infection. In this study we have modified
       the chromosome of L. monocytogenes so that it stably expresses and
       secretes the p55 HIV gag gene product and examined the cell-mediated
       immune response of BALB/c mice to infection with this recombinant
       organism. Infected animals were found to mount a specific, strong,
       long-lasting CD8+ cytolytic T cell response against a predominant
       epitope contained within the p24 fragment of the HIV Gag protein. This
       epitope previously has been shown to be recognized by CTLs obtained from
       some HIV-infected humans. Our results suggest that chromosomally
       modified strains of L. monocytogenes may provide valuable vaccine
       vectors for use against HIV.
 DE    Amino Acid Sequence  Animal  *AIDS Vaccines  Base Sequence
       Cytotoxicity, Immunologic  Gene Products, gag/*GENETICS/*IMMUNOLOGY
       Gene Transfer  Human  *HIV-1  *Immunity, Cellular  Listeria
       monocytogenes/*GENETICS/IMMUNOLOGY  Mice  Mice, Inbred BALB C  Molecular
       Sequence Data  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).