Document 0535
 DOCN  M9620535
 TI    Early activation signal transduction pathways of Th1 and Th2 cell clones
       stimulated with anti-CD3. Roles of protein tyrosine kinases in the
       signal for IL-2 and IL-4 production.
 DT    9602
 AU    Tamura T; Nakano H; Nagase H; Morokata T; Igarashi O; Oshimi Y; Miyazaki
       S; Nariuchi H; Department of Allergology, University of Tokyo, Japan.
 SO    J Immunol. 1995 Nov 15;155(10):4692-701. Unique Identifier : AIDSLINE
       MED/96062285
 AB    In the present experiments, TCR-CD3-associated early activation signal
       transduction pathways were examined in Th1 and Th2 clones by the
       stimulation with soluble monovalent anti-CD3 which resulted in efficient
       production of IL-2 and IL-4 in Th1 and Th2 cells, respectively. Although
       protein tyrosine kinases such as Fyn and ZAP-70 were activated in Th1
       clones shortly after stimulation, these kinases in Th2 clones were not
       activated; but, their activity in resting conditions was shown to be
       decreased by the stimulation. In accordance with these findings, neither
       phospholipase C-gamma 1 activation nor phosphatidyl
       inositol-4,5-bisphosphate breakdown was induced in Th2 clones, in
       contrast to positive responses in Th1 clones. The oscillation of
       intracellular free Ca2+ concentration ([Ca2+]i) was a common signal for
       the activation of both Th1 and Th2 clones; however, the [Ca2+]i
       elevation in Th1 clones was herbimycin A sensitive, whereas that in Th2
       was clone resistant, suggesting that the mechanism of the [Ca2+]i
       elevation in Th2 cells is different from that in Th1 cells in terms of
       the participation of protein tyrosine kinases. The anti-CD3 stimulation
       did not cause Lck activation in either the Th1 or Th2 clone, although
       remarkable activation was induced in both clones following anti-CD4
       stimulation, indicating that Lck activation was not required for either
       IL-2 or IL-4 production of Th cells. Taken together, these results
       indicate that Th1 and Th2 cells are different from each other in early
       activation signal transduction pathways, especially in the role of
       protein tyrosine kinases.
 DE    Animal  Antigens, CD3/*METABOLISM  Clone Cells  Enzyme Activation
       Interleukin-2/*BIOSYNTHESIS  Interleukin-4/*BIOSYNTHESIS  Mice  Mice,
       Inbred C3H  Mice, Inbred C57BL  Protein-Tyrosine Kinase/*METABOLISM
       Proto-Oncogene Proteins/*METABOLISM  *Signal Transduction  Support,
       Non-U.S. Gov't  Th1 Cells/*METABOLISM  Th2 Cells/*METABOLISM  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).