Document 0590
 DOCN  M9620590
 TI    Human T-cell-leukemia virus type I in post-transfusional spastic
       paraparesis: complete proviral sequence from uncultured blood cells.
 DT    9602
 AU    Bazarbachi A; Huang M; Gessain A; Saal F; Saib A; Peries J; De The H;
       Galibert F; UPR43 CNRS, Retrovirus et Retrotransposons des vertebres,;
       Hopital Saint-Louis, Paris, France.
 SO    Int J Cancer. 1995 Nov 15;63(4):494-9. Unique Identifier : AIDSLINE
       GENBANK/L36905
 AB    Human T-cell-leukemia virus type I (HTLV-I) is the causative agent of
       adult T-cell leukemia/lymphoma (ATL) and tropical spastic
       paraparesis/HTLV-I-associated myelopathy (TSP/HAM). The different
       disease outcome may be attributable to subtle mutations leading to
       modification of viral tropism or infectivity. Initial attempts found a
       very high level of sequence conservation among all HTLV-I strains.
       However, only one complete proviral DNA sequence is reported from a
       TSP/HAM patient, with a provirus derived from immortalized lymphocytes,
       which might be expected to be a leukemogenic variant rather than a
       neurotropic one. We cloned and sequenced a complete HTLV-I provirus
       (HTLV-IBoi) derived from the uncultured lymphocytes of a sub-acute
       post-transfusional TSP/HAM patient with clonal integration of HTLV-I.
       HTLV-IBoi proviral genome is 9033 bp long, and its overall genetic
       organization is similar to that of the prototype HTLV-I(ATK), without
       major deletions or insertions. No premature termination codon was found
       in the 4 open reading frames of the pX region. Divergence at the
       nucleotide level of HTLV-IBoi from the reported full-length HTLV-I
       varies from 1 to 9.4%, and indicates that it corresponds to a
       cosmopolitan genotype. This study did not identify specific sequences
       associated with neurotropic strains.
 DE    Adult  Alternative Splicing  Amino Acid Sequence  Base Sequence  Blood
       Transfusion/*ADVERSE EFFECTS  Case Report  Cloning, Molecular
       Comparative Study  Genes, Viral  Human  HTLV-I/*GENETICS  Leukocytes,
       Mononuclear/*VIROLOGY  Male  Molecular Sequence Data  Open Reading
       Frames  Paraparesis, Tropical Spastic/BLOOD/*VIROLOGY
       Proviruses/*GENETICS  Sequence Homology, Amino Acid  Sequence Homology,
       Nucleic Acid  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).