Document 0651
 DOCN  M9620651
 TI    A B7-1-transfected human melanoma line stimulates proliferation and
       cytotoxicity of autologous and allogeneic lymphocytes.
 DT    9602
 AU    Sule-Suso J; Arienti F; Melani C; Colombo MP; Parmiani G; Division of
       Experimental Oncology D, Istituto Nazionale Tumori,; Milan, Italy.
 SO    Eur J Immunol. 1995 Oct;25(10):2737-42. Unique Identifier : AIDSLINE
       MED/96062018
 AB    B7 co-stimulation is necessary to activate resting T cells upon antigen
       recognition by the T cell receptor. To see whether expression of B7 may
       render human melanoma cells able to stimulate T cells, a cloned melanoma
       line (Me1B6), which did not express B7-1, was transfected with the human
       B7-1 gene. In proliferation assays, B7-1 transfected cells (Me1B6/B7)
       showed greater stimulatory activity of allogeneic and autologous
       peripheral blood lymphocytes (PBL) compared to parental, non-transfected
       tumor cells. This effect was also seen when allogeneic CD8+ and CD4+
       subpopulations were used as effectors. In these studies, activation of
       lymphocytes was B7-1-dependent and HLA classes I and II mediated. The
       higher proliferation correlated with an increased lytic activity by PBL
       stimulated with B7-1+ tumor cells against the untransfected Me1B6.
       Furthermore, PBL from a metastatic melanoma patient stimulated by
       Me1B6/B7 developed an higher lytic activity not only against Me1B6 but
       also against their autologous, B7-1- tumor. Finally, after Me1B6/B7
       stimulation, PBL released interleukin (IL)-2 and interferon-gamma, but
       not IL-4, suggesting a Th1-mediated response. These data support the use
       of B7-1 transfected melanoma cells in the therapeutic vaccination of
       melanoma patients.
 DE    Antigens, CD80/GENETICS/*PHYSIOLOGY  Cell Division  Cells, Cultured
       Cytotoxicity Tests, Immunologic  *Cytotoxicity, Immunologic  Haplotypes
       Human  HLA Antigens/IMMUNOLOGY  Interferon Type II/SECRETION
       Interleukin-2/SECRETION  Lymphocyte Culture Test, Mixed  *Lymphocyte
       Transformation  Melanoma/*IMMUNOLOGY/PATHOLOGY  Neoplasm Metastasis
       Skin Neoplasms/*IMMUNOLOGY/PATHOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/*IMMUNOLOGY  Th1 Cells/IMMUNOLOGY  Transfection  Tumor
       Cells, Cultured  Vaccination  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).