Document 0653
 DOCN  M9620653
 TI    Detection of HIV-1 proviral sequences in lymphocytes using a qualitative
       polymerase chain reaction assay.
 DT    9602
 AU    Marin MG; Lillo F; Varnier OE; Bresciani S; Molinelli A; Abecasis C;
       Bonini PA; Albertini A; Institute of Chemistry, School of Medicine,
       University of; Brescia, Spedali Civili, Italy.
 SO    Eur J Clin Microbiol Infect Dis. 1995 Jul;14(7):621-5. Unique Identifier
       : AIDSLINE MED/96047093
 AB    The performance and clinical relevance of a qualitative PCR-based assay
       for the detection of HIV-1 DNA sequences in peripheral blood mononuclear
       cells (PBMCs) was evaluated by two different laboratories. Four hundred
       and one samples were obtained from 397 individuals from different risk
       populations. All blood donors tested had negative results; positive
       signals were obtained from all infected patients. HIV-1 DNA was detected
       in 3 of 17 infants born to seropositive mothers; Western blot
       indeterminate blood donors and exposed health-care workers had negative
       results. Our results demonstrate that this PCR assay provides both
       sensitive and specific results and is suitable for testing large numbers
       of samples and for rapid identification of HIV-1 infection.
 DE    Base Sequence  DNA, Viral/*ANALYSIS  Human  HIV-1/GENETICS/*ISOLATION &
       PURIF  Lymphocytes/*VIROLOGY  Molecular Sequence Data  *Polymerase Chain
       Reaction  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).