Document 0677
 DOCN  M9620677
 TI    Expression of the Fas antigen in patients infected with human
       immunodeficiency virus.
 DT    9602
 AU    McCloskey TW; Oyaizu N; Kaplan M; Pahwa S; Department of Pediatrics,
       North Shore University Hospital-Cornell; University Medical College,
       Manhasset, NY 11030, USA.
 SO    Cytometry. 1995 Jun 15;22(2):111-4. Unique Identifier : AIDSLINE
       MED/96090293
 AB    Lymphocytes from patients with HIV infection have been shown to undergo
       accelerated apoptosis. Fas antigen is a cell surface protein known to
       initiate an apoptotic signal. Therefore, we undertook a study to examine
       the expression of the Fas antigen during HIV infection. Using three
       color flow cytometry, expression of the Fas antigen on lymphocytes of 23
       HIV infected individuals (CDC category 2, CD4 200-499 cells/microL, n =
       10; CDC category 3, CD4 < 200 cells/microL, n = 13) and 10 healthy
       controls was examined. Both CD3+CD4+ and CD3+CD8+ subsets were examined
       for their expression of this marker. In lymphocytes of healthy controls,
       47% of the CD3+CD4+ and 45% of the CD3+CD8+ cells were Fas antigen
       positive. This percentage was significantly increased in CD4 cells from
       HIV infected patients belonging to CDC category 3, but was unchanged
       from normal values in CDC category 2 subjects. The increase in the
       percentage of CD4+ T cells expressing Fas antigen in patients correlated
       significantly with the decrease in circulating CD4 T cell count (P <
       0.009). In addition, by examining mean fluorescence intensity, we found
       that the amount of Fas expression per cell was increased threefold in
       CD3+CD4+ cells and increased twofold in CD3+CD8+ cells in category 3
       patients. These results demonstrate that an increase in Fas antigen
       expression occurs during HIV infection.
 DE    Adult  Aged  Antigens, CD95/*BIOSYNTHESIS  Case-Control Studies  CD4
       Lymphocyte Count  CD4-Positive T-Lymphocytes/*IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/*IMMUNOLOGY  Female  Human  HIV Antigens/*BIOSYNTHESIS
       HIV Infections/*IMMUNOLOGY  Male  Middle Age  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

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