Document 0809
 DOCN  M9620809
 TI    The effects of FK506 and dexamethasone on rat thymocyte differentiation.
 DT    9602
 AU    Tsuchida M; Takai K; Jojima K; Naito K; Fujikura Y; Fukumoto T;
       Department of Urology, Yamaguchi University School of Medicine,; Japan.
 SO    Ther Immunol. 1994 Jun;1(3):135-41. Unique Identifier : AIDSLINE
       MED/96053297
 AB    We investigated the effects of FK506 and glucocorticoids (GC) on rat
       thymocytes using flow cytofluorometry. Rats were treated with GC (0.1
       mg/body, by single injection), with FK506 (1 mg/kg/day, for 7 days), or
       with FK506 and GC. GC alone significantly decreased the percentage of
       CD4+8+ thymocytes and increased the percentages of CD4-8-, CD4+8- and
       CD4-8+ thymocytes on day 7. FK decreased the percentage of CD4+8- and
       CD4-8+ thymocytes and increased the percentage of CD4+8+ thymocytes on
       days 5 to 14. FK and GC induced a significant decrease in the number of
       CD4+8+ thymocytes greater than that seen with GC alone on day 7. The
       absolute number of TCR alpha beta high MHC class Ihigh thymocytes after
       FK and GC was significantly lower than that of the control group, and
       was slightly lower than that after FK alone on day 14. These results
       suggest that combined treatment with FK506 and GC acts complexly to
       decrease rat CD4+8+ thymocytes and prevents thymocyte differentiation
       and maturation.
 DE    Animal  Body Weight/DRUG EFFECTS  Cell Differentiation/DRUG EFFECTS
       CD4-Positive T-Lymphocytes/CYTOLOGY  CD8-Positive T-Lymphocytes/CYTOLOGY
       Dexamethasone/PHARMACOLOGY  Flow Cytometry  Histocompatibility Antigens
       Class I/METABOLISM  Immunosuppressive Agents/*PHARMACOLOGY  Male  Rats
       Rats, Inbred Lew  Receptors, Antigen, T-Cell, alpha-beta/METABOLISM
       T-Lymphocyte Subsets/*CYTOLOGY  Tacrolimus/*PHARMACOLOGY  Thymus
       Gland/*CYTOLOGY  JOURNAL ARTICLE

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