Document 0817
 DOCN  M9620817
 TI    A new reporter system for detection of retroviral infection.
 DT    9602
 AU    Planelles V; Haislip A; Withers-Ward ES; Stewart SA; Xie Y; Shah NP;
       Chen IS; Department of Medicine, UCLA School of Medicine, USA.
 SO    Gene Ther. 1995 Aug;2(6):369-76. Unique Identifier : AIDSLINE
       MED/96002912
 AB    We describe a novel reporter molecule, the murine surface antigen Thy-1,
       useful for immunoselection and detection of retrovirus-mediated
       transduction by flow cytometry. A cDNA encoding the murine thy-1 gene
       was isolated, and cell surface expression of its gene product was
       demonstrated. The Thy-1 glycoprotein was tested as a cell surface
       reporter molecule in the context of replication-defective and -competent
       retroviruses. Cells transduced via murine retroviral vectors carrying
       the thy-1 and the neomycin phosphotransferase genes express Thy-1
       glycoprotein on their surfaces. The Thy-1 marker is potentially useful
       in gene transfer protocols because selection of transduced cells can be
       achieved by immunoselection with anti-Thy-1 antibodies shortly after
       infection with the retroviral vector. In addition, a human
       immunodeficiency virus type 1 (HIV-1) recombinant expressing Thy-1 is
       described, which is replication-competent and syncytium-inducing in
       human peripheral blood mononuclear cells (PBMCs) and immortalized
       CD4-positive cell lines. Cells infected with this HIV-1 recombinant
       express Thy-1 on their surfaces and can be detected and purified by
       fluorescence-activated cell sorting (FACS). Because of these properties,
       retroviruses expressing this genetic marker can be useful for studies in
       gene therapy and of the retroviral life-cycle.
 DE    Animal  Antigens, Thy-1/ANALYSIS/*BIOSYNTHESIS  Base Sequence  Cell Line
       Cells, Cultured  Cercopithecus aethiops  CD4-Positive
       T-Lymphocytes/VIROLOGY  DNA Primers  DNA, Viral/ANALYSIS/*BIOSYNTHESIS
       Flow Cytometry  Gene Expression  Giant Cells  Human  HIV Core Protein
       p24/BIOSYNTHESIS  HIV Long Terminal Repeat  HIV-1/*GENETICS
       Lymphocytes/*VIROLOGY  Mice  Molecular Sequence Data
       Phosphotransferases (Alcohol Group Acceptor)/BIOSYNTHESIS  Polymerase
       Chain Reaction  Support, U.S. Gov't, P.H.S.  *Transfection  Tumor Cells,
       Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).