Document 0818
 DOCN  M9620818
 TI    Cell targeting with retroviral vector particles containing
       antibody-envelope fusion proteins.
 DT    9602
 AU    Chu TH; Martinez I; Sheay WC; Dornburg R; UMDNJ/Robert Wood Johnson
       Medical School, Department of; Microbiology, Piscataway 08854, USA.
 SO    Gene Ther. 1994 Sep;1(5):292-9. Unique Identifier : AIDSLINE
       MED/96050953
 AB    Retroviral vectors are the most efficient tool to introduce genes into
       vertebrate cells. However, their use is limited by the host range of the
       retrovirus from which they were derived. To alter the host range of the
       vector particle, we developed a method to substitute the
       receptor-binding domain of the envelope protein of a retrovirus with an
       antigen-binding site of an antibody. To test whether such particles are
       competent for infection, we established a model system using an
       antigen-binding site of an antibody against the hapten dinitrophenol
       (DNP). Retroviral vector particles containing such chimeric envelope
       proteins were able to bind to and infect cells that were not infectable
       with wild-type virus after DNP was conjugated to the cell surface. They
       did not infect such cells without DNP conjugation. Control experiments
       with chimeric envelope proteins of ecotropic murine leukemia virus
       (eco-MLV) and spleen necrosis virus (SNV) indicate that the pathway of
       virus entry of scA-env-containing virus particles was different from
       that of wild-type virus.
 DE    Animal  Antibodies/*GENETICS  Binding Sites, Antibody/GENETICS  Cell
       Line  CHO Cells  Dinitrophenols/IMMUNOLOGY  Gene Products, env/*GENETICS
       *Gene Targeting  *Genetic Vectors  Hamsters  Hela Cells  Human  Leukemia
       Viruses, Murine/GENETICS  Recombinant Fusion Proteins/GENETICS
       Retroviridae/*GENETICS  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).