Document 0853
 DOCN  M9620853
 TI    Neutralizing antibodies in slowly progressing HIV-1 infection.
 DT    9602
 AU    Schonning K; Nielsen C; Iversen J; Nielsen JO; Hansen JE; Department of
       Infectious Diseases, Hvidovre Hospital, Denmark.
 SO    J Acquir Immune Defic Syndr Hum Retrovirol. 1995 Dec 1;10(4):400-7.
       Unique Identifier : AIDSLINE MED/96074272
 AB    Ten asymptomatic individuals who had experienced only limited CD4+ cell
       loss after prolonged infection with HIV-1 were studied. These
       individuals had a mean CD4+ cell count of 674 x 10(6) cells/L and a mean
       duration of infection of 8.5 years. Also included were 10 asymptomatic
       HIV-1-infected individuals who, over a similar period of infection (7.5
       years), had experienced a profound loss of CD4+ cells (mean CD4+ cell
       count, 54 x 10(6) cells/L). Proviral load was determined using a
       semiquantitative polymerase chain reaction and was significantly lower
       in the subjects with slowly progressing infection (SPI) than in subjects
       with rapidly progressing infection (RPI) (4,000 vs. 40,000 proviral
       copies/10(6) peripheral blood mononuclear cells; p = 0.0089). Isolation
       of virus was attempted in all individuals but succeeded only in 6 of 10
       individuals with SPI versus all 10 individuals with RPI. Four of six
       virus isolates obtained from individuals with SPI and 6 of 10 obtained
       from individuals with RPI were of the syncytia-inducing phenotype. We
       determined the neutralizing activity of serum against HIVMN, HIVIIIB,
       and the contemporaneous autologous isolate when available. Serum from
       individuals with SPI generally neutralized the contemporaneous isolate,
       whereas serum from individuals with RPI did not [geometric mean antibody
       titer (GMT), 45 vs. 3; p = 0.0047]. There was no difference in
       neutralizing ability against HIVMN (GMT,2,593 vs. 2,263; p = 0.74) and
       only a small difference against HIVIIIB (GMT, 115 vs. 50; p = 0.075).
       Our results indicate that individuals with SPI are characterized by an
       ability to neutralize their own HIV strain.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Adult  Base Sequence  Cell Fusion  CD4 Lymphocyte Count  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY  Disease Progression  DNA Primers/CHEMISTRY
       DNA, Viral/ANALYSIS  Enzyme-Linked Immunosorbent Assay  Gene Products,
       pol/IMMUNOLOGY  Human  HIV Antibodies/*IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY/PHYSIOPATHOLOGY  HIV-1/*IMMUNOLOGY/ISOLATION &
       PURIF  Male  Middle Age  Molecular Sequence Data  Neutralization Tests
       Polymerase Chain Reaction  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).