Document 0946
 DOCN  M9620946
 TI    Identification of Mycobacterium intracellulare by a polymerase chain
       reaction using species-specific primers.
 DT    9602
 AU    Yamazaki T; Nakamura RM; Department of Bacteriology, National Institute
       of Health, Tokyo,; Japan.
 SO    Tuber Lung Dis. 1995 Aug;76(4):330-5. Unique Identifier : AIDSLINE
       MED/96048197
 AB    SETTING: The polymerase chain reaction (PCR) is a rapid and specific
       method used to amplify a certain DNA fragment. It is applicable to rapid
       diagnosis of mycobacterial infections. By use of species-specific
       primers, it is possible to identify mycobacteria by PCR. In this study,
       a newly constructed primer was tested for specificity for Mycobacterium
       intracellulare in the PCR. OBJECTIVE: M. intracellulare is one of the
       most frequently found bacteria in opportunistic infection in AIDS, and
       rapid identification of this species is important. The purpose of this
       study was to construct a primer specific to this species as a suitable
       tool for identification. DESIGN: PCR products of M. tuberculosis and M.
       intracellulare, obtained by using the primers YNP-1 and YNP-2, were
       sequenced and compared. They showed a difference in the base sequences.
       A sequence unique to M. intracellulare was used as the primer specific
       to this species. Various mycobacterial and non-mycobacterial DNAs were
       used as the primer specific to this species. Various mycobacterial and
       non-mycobacterial DNAs were used as the template to evaluate the
       specificity of the newly constructed primers, YNP-7 and YNP-8. Sputum
       samples were also examined by PCR using the primers. RESULTS: In total
       25 species of culture mycobacterial and non-mycobacterial strains and 76
       sputum samples were tested by PCR. Only M. intracellulare DNA was
       amplified with PCR using the primers YNP-7/8. CONCLUSION: The
       specificity of the newly constructed primers for M. intracellulare was
       confirmed.
 DE    *Bacterial Typing Techniques  Base Sequence  DNA Primers  DNA,
       Bacterial/*ANALYSIS  Human  Molecular Sequence Data
       Mycobacterium/CLASSIFICATION  Mycobacterium avium Complex/*ISOLATION &
       PURIF  Mycobacterium avium-intracellulare Infection/DIAGNOSIS
       Polymerase Chain Reaction/*METHODS  Species Specificity
       Sputum/MICROBIOLOGY  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).