Document 1034
 DOCN  M9621034
 TI    Seroreactivity of analogous antigenic epitopes in glycoprotein 120
       expressed in HIV-1 subtypes A, B, C, and D.
 DT    9602
 AU    Pestano GA; Hosford KS; Spira AI; Riley J; Xie JM; Sewankambo N; Brown
       L; Ho DD; Boto WM; Department of Biology, City College of the City
       University of New; York, New York 10031, USA.
 SO    AIDS Res Hum Retroviruses. 1995 May;11(5):589-96. Unique Identifier :
       AIDSLINE MED/96093894
 AB    This article describes the impact of sequence variation on the
       distribution and seroreactivity of linear antigenic epitopes in gp120
       encoded in new Ugandan HIV-1 clones from subtypes A, C, and D, and in
       North American clones from the B subtype. A region of the env gene
       encoding the C2 to V5 domains was PCR amplified from the lysates of
       peripheral blood leukocytes or from short-term cultured isolates.
       Computer-assisted analyses were conducted on the amino acid sequences to
       determine the distribution of surface structures in gp120. Despite
       marked sequence diversity, eight analogous epitopes were predicted for
       all clades of the virus analyzed. Synthetic peptides comprising the
       putative principal neutralizing determinant E2[V3], and other B cell
       epitopes E3[V3-V4], E4[V3-V4], E7[C3], and E8[V5], from a seroprevalent
       Ugandan isolate, AUG06c, were tested in ELISA for antigenicity with sera
       from Uganda, New York, and Thailand. Variable magnitudes of
       seroreactivity were observed for all of the peptides tested. However, a
       significantly higher degree of serum cross-reactivity was detected with
       the V3 loop peptide. ELISA reactivities of the same serum panel
       indicated that V3 loop peptides containing the apical residues GPGR
       (clones AUG06c and BRT3) or GPGQ (CUG045 and DUG044) were more antigenic
       and display extensive cross-reactivity as compared to analogous peptides
       comprising GLGQ (DUG23c), GQGQ (DUG042), or GPWG (BRT1). BETATURN
       analysis of the divergent V3 loop apical residues showed a good
       correlation of probable beta-turn occurrence with strong seroreactivity.
       These findings suggest that the major antigenic specificities in the
       divergent clades of HIV-1 are well conserved.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Amino Acid Sequence  Antigenic Variation  Epitopes/GENETICS/*IMMUNOLOGY
       Genes, env  Human  HIV Antigens/GENETICS/*IMMUNOLOGY  HIV Envelope
       Protein gp120/GENETICS/*IMMUNOLOGY  HIV Seropositivity/BLOOD/VIROLOGY
       HIV-1/CLASSIFICATION/GENETICS/*IMMUNOLOGY  Molecular Sequence Data  New
       York  Peptide Fragments/IMMUNOLOGY  Sequence Homology, Amino Acid
       Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Support, U.S.
       Gov't, P.H.S.  Thailand  Uganda  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).