Document 1042
 DOCN  M9621042
 TI    Detection of a 67-kD glycoprotein in human tumor cell lines by a
       monoclonal antibody established against a recombinant human endogenous
       retrovirus-K envelope-gene-encoded protein.
 DT    9602
 AU    Vogetseder W; Feng J; Haibach C; Mayerl W; Dierich MP; Institut fur
       Hygiene, Leopold-Franzens-Universitat Innsbruck,; Osterreich.
 SO    Exp Clin Immunogenet. 1995;12(2):96-102. Unique Identifier : AIDSLINE
       MED/96100697
 AB    Endogenous retroviruses in humans are discussed as putative etiologic
       agents in tumorigenesis. Molecular mimicry of cellular epitopes by
       retroviruses may be of importance for the genesis of autoimmune
       diseases. A group of human endogenous retroviruses, HERV-K, which is
       related to the mouse mammary tumour virus, has been characterised
       previously and open reading frames have been found covering their gag,
       pol, prt and env genes. Transcription of HERV-K genomes at the mRNA
       level as well as antibodies in human sera directed against HERV-K env
       have been detected recently. We have generated a monoclonal antibody
       (mAb), anti-HERV1, against a recombinant HERV-K Env protein. This mAb is
       capable of immunoprecipitating a 67-kD glycoprotein from the human
       breast carcinoma cell line T47D, the amount of which is strongly
       enhanced after stimulation with estradiol followed by progesterone. The
       same band could be precipitated from other carcinoma cell lines (Hep2,
       MCF7 and HeLa), but not from the human B-lymphoblastoid cell line Raji
       and from cultured human fibroblasts. Incubation of this antigen in the
       presence of endoglycosidase F indicates the presence of N-linked
       carbohydrate moieties of at least 7-9 kD.
 DE    Animal  Antibodies, Monoclonal/BIOSYNTHESIS/*CHEMISTRY  Carbohydrate
       Conformation  Gene Products, env/CHEMISTRY/*IMMUNOLOGY  Genes,
       env/IMMUNOLOGY  Human  Mice  Mice, Inbred BALB C  Molecular Weight
       Precipitin Tests  Recombinant Proteins/IMMUNOLOGY
       Retroviridae/GENETICS/*IMMUNOLOGY  Support, Non-U.S. Gov't  Tumor Cells,
       Cultured/*IMMUNOLOGY  JOURNAL ARTICLE

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