Document 0922
 DOCN  M9650922
 TI    In vitro response of lymphocytes from bronchoalveolar lavage fluid and
       peripheral blood to mitogen stimulation during natural maedi-visna virus
       infection.
 DT    9505
 AU    Begara I; Lujan L; Collie DS; Miller HR; Watt NJ; Department of
       Veterinary Clinical Studies, Royal (Dick) School of; Veterinary Studies,
       University of Edinburgh, Easter Bush,; Midlothian, UK.
 SO    Vet Immunol Immunopathol. 1995 Nov;49(1-2):75-88. Unique Identifier :
       AIDSLINE MED/96158378
 AB    To investigate the effects of maedi-visna virus (MVV) infection on
       cell-mediated immunity, the in vitro response of bronchoalveolar lavage
       fluid (BALF) and peripheral blood (BP) lymphocytes (PBL) to exogenous
       mitogen was analysed. BALF and PBL from control (n = 9) and MVV-infected
       (n = 7) animals were cultured fro 3 days in the presence and absence of
       concanavalin A (Con A). Lymphocyte expression of the interleukin-2
       receptor (IL-2R) antigen, a parameter of lymphocyte activation, was
       quantified by dual-colour flow cytometry using the bovine anti-IL-2R
       monoclonal antibody IL-A111. IL-2R expression by lymphocytes in BALF and
       PB from control and MVV-infected animals, with and without Con A
       stimulation, were compared. In the absence of Con A stimulation, the
       proportion of cultured BALF CD8+ and gamma delta T cells expressing
       IL-2R was significantly (P < 0.05) lower for MVV-infected animals than
       for controls. After Con A stimulation the proportion of BALF CD4+
       lymphocytes from MVV-infected animals that expressed IL-2R remained
       significantly (P < 0.05) lower than for controls. Comparisons within
       group showed that, after Con A stimulation, the proportion of all the T
       cell subsets in the control group expressing IL-2R, namely CD4+ (P <
       0.001), CD8+ (P < 0.001) and gamma delta T cells (P < 0.05), was
       significantly increased. In the MVV-infected group, this increase was
       significant (P < 0.05) for CD4+ and CD8+ T cells, but not for gamma
       delta T cells. In vitro mitogen stimulation of PB T lymphocytes from
       both control and MVV-infected animals induced a significant elevation in
       the proportion of all T cell subsets expressing IL-2R when compared to
       cultured unstimulated control cells. However, there was considerable
       heterogeneity in the response to Con A of PB T cells from both groups of
       animals. The expression of IL-2R followed a different pattern to that of
       BALF lymphocytes, the proportion of unstimulated gamma delta / IL-2R+ T
       cells from MVV-infected animals being significantly (P < 0.05) higher
       than that of controls, and the proportion of cultured unstimulated CD8+
       / IL-2R+ T cells from MVV-infected animals being significantly (P <
       0.05) lower than that from controls. From these studies it can be
       concluded that the BALF T lymphocyte immune dysfunction observed during
       natural MVV infection, characterized by impaired IL-2R expression, is
       maintained under in vitro conditions.
 DE    Animal  Bronchoalveolar Lavage Fluid/CYTOLOGY/*IMMUNOLOGY  Cattle
       Concanavalin A/PHARMACOLOGY  CD4-Positive T-Lymphocytes/IMMUNOLOGY
       CD8-Positive T-Lymphocytes/IMMUNOLOGY  Female  In Vitro  *Lymphocyte
       Transformation  Lymphocytes/*IMMUNOLOGY  Macrophages,
       Alveolar/IMMUNOLOGY  Pneumonia, Progressive Interstitial, of
       Sheep/*IMMUNOLOGY  Receptors, Antigen, T-Cell, gamma-delta/METABOLISM
       Receptors, Interleukin-2/METABOLISM  Sheep  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).