Document 0925
 DOCN  M9650925
 TI    [Detection of human immunodeficiency virus antigen both free and in
       immune complexes]
 DT    9505
 AU    Banfi NH; Minervini MV; Scoccia AE; Servicio de Laboratorio Central,
       Hospital I.A.C. San Juan de; Dios, La Plata, Argentina.
 SO    Rev Argent Microbiol. 1995 Jul-Sep;27(3):123-9. Unique Identifier :
       AIDSLINE MED/96124262
 AB    The aim of this work was to increase sensitivity in the detection of
       antigens from HIV-infected patients, through a process of immune complex
       dissociation without loss of antigenicity. 500 microliters of sera were
       mixed with 100 microliters of PEG 12%, stored one night in refrigerator,
       and centrifuged at 2000 g during 20 minutes. 200 microliters of buffer
       AcH/Ac- (pH 3.5) were added to the sediment, and incubated at 37 degrees
       C during one hour with periodic shaking. This was neutralized with 100
       microliters of buffer TRIS/CIH (pH 8.6). The antigen was investigated in
       the original sample, supernatant and sediment. Samples of 105 patients
       with positive serology, confirmed by Western Blot following CDC
       criteria, were processed. The antigen was detected in 62 (59%) samples
       precipitated with PEG, but only 35 (33%) when conventional methods were
       used. Applying statistics X2: 13.97, P < 0.001, a highly significant
       association can be observed between PEG dissociation treatment and
       antigen detection. 27 negative sera by the standard method became
       positive in the whole sediment, and only 8 in the supernatant. In
       addition, 40 negative sera were processed, which had not become positive
       for the antigen by PEG treatment.
 DE    Antigen-Antibody Complex/*IMMUNOLOGY  English Abstract  False Negative
       Reactions  Human  HIV Antigens/*BLOOD  HIV Infections/BLOOD/IMMUNOLOGY
       HIV Seronegativity  HIV Seropositivity/BLOOD  HIV-1/*IMMUNOLOGY
       Polyethylene Glycols  Precipitation  Reference Standards  Sensitivity
       and Specificity  JOURNAL ARTICLE

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