Document 0927
 DOCN  M9650927
 TI    Development and ultrastructure of Trachipleistophora hominis n.g., n.sp.
       after in vitro isolation from an AIDS patient and inoculation into
       athymic mice.
 DT    9505
 AU    Hollister WS; Canning EU; Weidner E; Field AS; Kench J; Marriott DJ;
       Department of Biology, Imperial College of Science, Technology; and
       Medicine, London, UK.
 SO    Parasitology. 1996 Jan;112 ( Pt 1):143-54. Unique Identifier : AIDSLINE
       MED/96155214
 AB    Continuous culture was achieved in several cell lines of a
       microsporidium obtained from the skeletal muscle of an AIDS patient.
       Development in COS-1 and RK13 cells was prolific. Spores from the
       original biopsy were also inoculated into athymic mice by i.m. and i.p.
       routes. Infection was found in several organs as well as in skeletal
       muscle after a few weeks. All stages were surrounded by an
       electron-dense surface coat. Meronts had 2-4 nuclei and divided by
       binary fission. In sporogony the surface coat became separated from the
       plasma membrane to form a sporophorous vesicle, within which division
       into sporoblasts was effected by repeated binary fissions. The number of
       sporoblasts (and later spores) within the sporophorous vesicles varied
       from 2 to > 32 and the sizes of the vesicles varied, according to the
       number of spores contained therein, from 5 microns diameter to 14.0 x
       11.0 microns. Spores measured 4.0 x 2.4 microns and had a prominent
       posterior vacuole. The parasite differs from the genus Pleistophora in
       that it does not form multinucleate sporogonial plasmodia and that the
       sporophorous vesicle enlarges during sporogony and its wall is not a
       multilayered structure. It is proposed to place it in a new genus and
       species Trachipleistophora hominis n.g., n.sp.
 DE    Animal  AIDS-Related Opportunistic Infections/*PARASITOLOGY  Cell Line
       Human  Mice  Mice, Inbred BALB C  Mice, Nude  Microspora
       Infections/COMPLICATIONS/*PARASITOLOGY
       Microsporida/CLASSIFICATION/GROWTH & DEVELOPMENT/*ISOLATION &
       PURIF/ULTRASTRUCTURE  Muscles/CYTOLOGY/PARASITOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).