Document 0929
 DOCN  M9650929
 TI    Roles for both CD4+ and CD8+ T cells in protective immunity against
       Onchocerca lienalis microfilariae in the mouse.
 DT    9505
 AU    Folkard SG; Bianco AE; Liverpool School of Tropical Medicine, UK.
 SO    Parasite Immunol. 1995 Oct;17(10):541-53. Unique Identifier : AIDSLINE
       MED/96164309
 AB    Mice inoculated with microfilariae of the filarial nematode Onchocerca
       lienalis clear their parasites from the skin over a period of 3 to 4
       months and are highly resistant to a challenge infection. The adoptive
       transfer of spleen cells at various time points following primary and
       secondary infections of mice shows that exposures of 50 days or greater
       are required for the generation of lymphocytes capable of transferring
       protection to naive recipients. This adoptive transfer of protection
       with spleen cells from infection-primed mice partitions with the T
       lymphocyte population. In contrast, the passive transfer of protection
       with spleen-derived B cells, or sera taken at various time points
       following infection was not achieved. Moreover, there was no detectable
       synergistic effect when B and T cells were co-administered to recipient
       animals. Depletion of CD4+ and CD8+ T cells with monoclonal antibodies
       shows that CD8+ T cells have some regulatory effect on parasite
       establishment early in primary infection, but this is later superseded
       by CD4+ T cell reactivity that is predominant both when primary
       infection microfilariae are cleared and also during resistance to
       reinfection. Measurement of cytokines in the sera of mice undergoing
       primary and secondary infections support a microfilariae-induced Th2
       activity, with high levels of IL-5 that are sustained upon reinfection,
       and low levels of IFN-gamma that are negligible at the time when mice
       are most strongly immune.
 DE    Animal  Antibodies, Monoclonal  Antilymphocyte Serum
       B-Lymphocytes/IMMUNOLOGY  CD4-Positive T-Lymphocytes/*IMMUNOLOGY
       CD8-Positive T-Lymphocytes/*IMMUNOLOGY  Immunotherapy, Adoptive
       Interferon Type II/BLOOD  Interleukin-4/BLOOD  Interleukin-5/BLOOD  Male
       Mice  Mice, Inbred BALB C  Mice, Inbred CBA  Microfilaria/IMMUNOLOGY
       Onchocerca/*IMMUNOLOGY  Onchocerciasis/*IMMUNOLOGY  Spleen/IMMUNOLOGY
       Support, Non-U.S. Gov't  Time Factors  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).