Document 0940
 DOCN  M9650940
 TI    Evaluation of a dipstick method for the detection of human
       immunodeficiency virus infection.
 DT    9505
 AU    Beristain CN; Rojkin LF; Lorenzo LE; Research and Biotechnology Center,
       Rosario, Argentina.
 SO    J Clin Lab Anal. 1995;9(6):347-50. Unique Identifier : AIDSLINE
       MED/96159704
 AB    Serology has been a fundamental tool to prevent post-transfusional
       infection with human immunodeficiency virus (HIV) and for
       epidemiological surveys, the first step to attempt control of the
       pandemia. Enzyme immunoassay is in widespread use. Nevertheless, simpler
       methods are needed in many countries, where laboratory facilities and
       trained personnel are limited, and HIV prevalence is high. The
       evaluation of a simple and noninstrumented HIV antibody test is
       presented here. The test employs synthetic antigens of HIV-1 and HIV-2
       attached to the teeth of a polystyrene comb, which fit into the wells of
       standard microtiter plates where samples are diluted. Captured
       antibodies are developed with colloidal gold-labeled Protein A. Three
       seroconversion panels plus 662 samples were tested, including HIV-1 and
       HIV-2-infected individuals, normal blood donors, and a noninfected baby
       born to a seroreactive mother. When compared with enzyme-linked
       immunosorbent assay (ELISA) and Western blot, the dipstick showed 100%
       sensitivity and 98.7% specificity. The simplicity of result evaluations
       and excellent reagent stability make the dipstick suitable for small
       blood banks and for epidemiological surveys.
 DE    Amino Acid Sequence  AIDS Serodiagnosis/*METHODS  Blotting, Western
       Comparative Study  Disease Transmission, Vertical  Enzyme-Linked
       Immunosorbent Assay  Evaluation Studies  Female  Human  HIV
       Antibodies/*BLOOD  HIV Antigens/GENETICS  HIV
       Infections/COMPLICATIONS/*DIAGNOSIS/TRANSMISSION  HIV
       Seropositivity/DIAGNOSIS  HIV-1/IMMUNOLOGY  HIV-2/IMMUNOLOGY  Indicators
       and Reagents  Infant  Infant, Newborn  Maternal-Fetal Exchange
       Molecular Sequence Data  Pregnancy  Pregnancy Complications, Infectious
       JOURNAL ARTICLE

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