Document 0949
 DOCN  M9650949
 TI    Molecular subtyping of human T-cell lymphotropic virus type 2 by
       single-strand conformation polymorphism analysis. Retrovirus
       Epidemiology Donor Study Group.
 DT    9505
 AU    Heneine W; Switzer WM; Busch M; Khabbaz RF; Kaplan JE; Retrovirus
       Diseases Branch, Centers for Disease Control and; Prevention, Atlanta,
       Georgia 30333, USA.
 SO    J Clin Microbiol. 1995 Dec;33(12):3260-3. Unique Identifier : AIDSLINE
       MED/96156139
 AB    Molecular subtyping of human T-cell lymphotropic virus type 2 (HTLV-2)
       by the currently used method of restriction fragment length polymorphism
       analysis may not be sufficiently discriminatory for transmission studies
       because of the predominance of single restriction types in various
       HTLV-2-infected populations. The utility of single-strand conformations
       polymorphism (SSCP) analysis was evaluated as a tool to improve the
       sensitivity of the subtyping of HTLV-2. The assay was designed to target
       a highly variable region in the long terminal repeat and was shown to be
       able to detect single nucleotide changes in cloned HTLV-2 sequences.
       Analysis of 52 HTLV-2 samples, of which 32 were from 16 sex partner
       pairs (16 males, 16 females), showed nine different SSCP patterns.
       Identical SSCP results were obtained for each of the 16 couples,
       suggesting the presence of similar viral genotypes and, therefore,
       supporting the likelihood of sexual transmission of HTLV-2 in each of
       these couples. Furthermore, SSCP analysis of seven HTLV-2 samples of the
       same restriction type (b5) showed five different SSCP patterns.
       Nucleotide sequencing of two samples with distinct SSCP patterns
       confirmed the sequence differences. SSCP provides a facile and
       discriminatory tool for the differentiation of HTLV-2 strains, including
       those previously indistinguishable by restriction fragment length
       polymorphism.
 DE    Base Sequence  Comparative Study  DNA Primers/GENETICS  DNA,
       Viral/GENETICS  Female  Human
       HTLV-II/*CLASSIFICATION/*GENETICS/ISOLATION & PURIF  HTLV-II
       Infections/TRANSMISSION/VIROLOGY  Male  Molecular Sequence Data
       Polymerase Chain Reaction  Polymorphism, Restriction Fragment Length
       *Polymorphism, Single-Stranded Conformational  Reproducibility of
       Results  Sensitivity and Specificity  Sequence Homology, Nucleic Acid
       Sexual Partners  Virology/*METHODS/STATISTICS & NUMER DATA  JOURNAL
       ARTICLE

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