Document 0974
 DOCN  M9650974
 TI    Cytokine response and polymerase chain reaction study of peripheral
       blood mononuclear cells in infants with human cytomegalovirus infection.
 DT    9505
 AU    Asanuma H; Numazaki K; Nagata N; Chiba S; Department of Pediatrics,
       School of Medicine, Sapporo Medical; University, Japan.
 SO    FEMS Immunol Med Microbiol. 1995 Oct;12(2):153-8. Unique Identifier :
       AIDSLINE MED/96163862
 AB    We tried to detect human cytomegalovirus (HCMV) DNA in CD4+ and CD8+ T
       lymphocytes from fourteen infants with HCMV hepatitis using polymerase
       chain reaction (PCR) assay. HCMV was isolated from their urine and
       anti-HCMV IgM antibody was detected in their sera. One set of primers
       were designed from a region--a major immediate early (IE) gene. We
       detected HCMV IE DNA in the specimens obtained from six infants. HCMV IE
       DNA was detected from CD4+ cells in two cases and from CD8+ cells in
       one. In three cases, HCMV IE DNA was detected from both CD4+ and CD8+
       cells. We also studied the relationship between HCMV infection and serum
       levels of cytokines. We determined serum levels of interleukin-4 (IL-4),
       tumor necrosis factor alpha (TNF-alpha) and soluble interleukin 2
       receptor (sIL-2R) which were associated with the activation of T
       lymphocytes by enzyme immunoassay. In the acute phase of HCMV infection,
       titers of sIL-2R were correlated with serum levels of liver enzymes in
       some cases. IL-4 and TNF-alpha activities were not detected in sera. It
       is likely that expression of viral genome on T lymphocytes as well as
       activities of some cytokines are associated with active HCMV infection.
 DE    Base Sequence  Cytokines/*BLOOD  Cytomegalovirus/*GENETICS/*ISOLATION &
       PURIF  Cytomegalovirus Infections/*IMMUNOLOGY/*VIROLOGY  CD4-Positive
       T-Lymphocytes/IMMUNOLOGY/VIROLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY/VIROLOGY  DNA Primers/GENETICS  DNA,
       Viral/BLOOD/GENETICS  Female  Hepatitis, Viral,
       Human/*IMMUNOLOGY/*VIROLOGY  Human  In Vitro  Infant
       Interleukin-4/BLOOD  Leukocytes, Mononuclear/*IMMUNOLOGY/*VIROLOGY  Male
       Molecular Sequence Data  Polymerase Chain Reaction  Receptors,
       Interleukin-2/METABOLISM  Support, Non-U.S. Gov't  Time Factors  Tumor
       Necrosis Factor/METABOLISM  JOURNAL ARTICLE

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