SPIDER

SPIDER (System for Processing if Image Data from Electron microscopy and Related fields) is a large command drivern image processing system for electron microscopy developed over 19 years. SPIDER performs conventional image processing operations on images and volumes. However, the main emphasis is on three-dimensional reconstruction from single particle images or from images obtained by tilting thick sections. The system combines methods from X-ray crystallography, statical optics, electrical engineering, graphics to achieve signal enhancement and reconstruction in situations with low signal to noise ratios. A separate point-and click viewer handles 3D visualization. Includes source code.

Joachim Frank

Research Scientist