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Cancer - Principles & Practice of Oncology
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1999-06-07
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482b
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8 lines
FIGURE 60-3. Production of retroviral vectors with packaging cells.
The gene of interest is cloned into a retroviral vector (see Fig.
60-2); and then is transfected into a helper cell line, which provides
the retroviral structural genes in trans. The retroviral structural
genes cannot be packaged due to the absence of a packaging sequence
(psi), while the retroviral vector can be packaged, thereby producing
a replication incompetent retrovirus. [ref: 187]